Differential modulation of innate immunity in vitro by probiotic strains of Lactobacillus gasseri.
Differential modulation of innate immunity in vitro by probiotic strains of Lactobacillus gasseri.
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DOI：
10.1186/1471-2180-13-298
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发表时间：
2013-12-23
期刊：
影响因子：
4.2
通讯作者：
Rossi M
中科院分区：
文献类型：
--
作者：
Luongo D;Miyamoto J;Bergamo P;Nazzaro F;Baruzzi F;Sashihara T;Tanabe S;Rossi M
不同种类的益生菌似乎对肠道免疫反应有不同的调节作用。此外，我们已经表明，在属于同一物种的益生菌菌株之间可以发现不同的免疫调节能力。在本研究中，我们进一步解决了这一问题，同时研究了L. gasseri，一种在人类患者中诱导相关免疫活性的物种。我们检测了两株L. gasseri， OLL2809和L13-Ia在小鼠骨髓源性树突状细胞（dc）和MODE-K细胞中改变细胞表面抗原表达、细胞因子产生和核红细胞2-相关因子2 （Nrf2）介导的细胞保护的能力，它们代表了肠细胞模型。观察了不同菌株对成熟树突状细胞表面标记物表达的差异影响；然而，两株菌株均显著诱导IL-12、TNF-α和IL-10的产生。通过分析MHC II分子的表达和IL-6的分泌，在MODE-K细胞中也显示出对OLL2809和L13-Ia的不同反应；然而，两种菌株均能提高细胞内谷胱甘肽水平。用MODE-K单层培养基处理未成熟的DC，通过下调共刺激标记物的表达和改变细胞因子谱，提高了细胞保护作用，并改变了DC成熟过程。值得注意的是，细菌条件下的MODE-K细胞培养基抑制了所检测细胞因子的表达，而细胞保护防御仅在暴露于oll2809条件培养基中的dc中显著增强。这些作用基本上是由分泌的细菌代谢物介导的。我们已经证明了L. gasseri菌株具有独特的能力来调节体外DCs和肠细胞。特别是，我们的研究结果强调了L. gasseri分泌的代谢物影响肠细胞- dc串扰的潜力。通过选定的益生菌菌株调节先天免疫的细胞机制可能有助于这些细菌在肠道内稳态中的有益作用。
Probiotics species appear to differentially regulate the intestinal immune response. Moreover, we have shown that different immune-modulatory abilities can be found among probiotic strains belonging to the same species. In this study, we further addressed this issue while studying L. gasseri, a species that induces relevant immune activities in human patients. We determined the ability of two strains of L. gasseri, OLL2809 and L13-Ia, to alter cell surface antigen expression, cytokine production and nuclear erythroid 2-related factor 2 (Nrf2)-mediated cytoprotection in murine bone marrow-derived dendritic cells (DCs) and MODE-K cells, which represent an enterocyte model. Differential effects of L. gasseri strains were observed on the expression of surface markers in mature DCs; nevertheless, both strains dramatically induced production of IL-12, TNF-α and IL-10. Distinctive responses to OLL2809 and L13-Ia were also shown in MODE-K cells by analyzing the expression of MHC II molecules and the secretion of IL-6; however, both L. gasseri strains raised intracellular glutathione. Treatment of immature DCs with culture medium from MODE-K monolayers improved cytoprotection and modified the process of DC maturation by down-regulating the expression of co-stimulatory markers and by altering the cytokine profile. Notably, bacteria-conditioned MODE-K cell medium suppressed the expression of the examined cytokines, whereas cytoprotective defenses were significantly enhanced only in DCs exposed to OLL2809-conditioned medium. These effects were essentially mediated by secreted bacterial metabolites. We have demonstrated that L. gasseri strains possess distinctive abilities to modulate in vitro DCs and enterocytes. In particular, our results highlight the potential of metabolites secreted by L. gasseri to influence enterocyte-DC crosstalk. Regulation of cellular mechanisms of innate immunity by selected probiotic strains may contribute to the beneficial effects of these bacteria in gut homeostasis.
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影响因子：
7.4
作者：
Lin, Patricia W.;Myers, Loren E. S.;Ray, Laurie;Song, Shuh-Chyung;Nasr, Tala R.;Berardinelli, Andrew J.;Kundu, Kousik;Murthy, Niren;Hansen, Jason M.;Neish, Andrew S.
通讯作者：
Neish, Andrew S.
影响因子：
4.4
作者：
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通讯作者：
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2.2
作者：
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Kaminogawa, Shuichi
影响因子：
4
作者：
D'Arienzo, R.;Bozzella, G.;Sisto, A.
通讯作者：
Sisto, A.
影响因子：
2.2
作者：
Lutz, MB;Kukutsch, N;Schuler, G
通讯作者：
Schuler, G