Epidermal Langerhans cells rapidly capture and present antigens from C-type lectin-targeting antibodies deposited in the dermis.
Epidermal Langerhans cells rapidly capture and present antigens from C-type lectin-targeting antibodies deposited in the dermis.
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DOI：
10.1038/jid.2009.343
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发表时间：
2010-03
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影响因子：
--
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抗原呈递细胞可以捕获沉积在皮肤中的抗原，包括皮下注射的疫苗。这些包括不同的树突状细胞（DC），例如表皮朗格汉斯细胞（LC）、真皮DC和真皮朗格兰+ DC。为了评估皮肤抗原对皮肤DC的访问，我们使用了针对两种C型凝集素内吞受体DEC-205/CD 205和langerin/CD 207的mAb。当应用于小鼠和人皮肤外植体培养物时，这些mAb被表皮LC有效地吸收。此外，抗DEC-205靶向langerin+ CD 103+和langerin− CD 103 −小鼠真皮DC。出乎意料的是，皮内注射任一种mAb，而不是同种型对照，导致LC在原位的强烈和快速标记，这意味着大分子可以通过基底膜扩散到表皮中。卵清蛋白偶联的抗DEC-205在体内靶向的表皮LC有效地将抗原呈递给CD 4+和CD 8 + T细胞。因此，表皮LC在标准接种条件下凝集素结合配体的摄取中起主要作用。
Antigen-presenting cells can capture antigens that are deposited in the skin, including vaccines given subcutaneously. These include different dendritic cells (DC) such as epidermal Langerhans cells (LC), dermal DC and dermal langerin+ DC. To evaluate access of dermal antigens to skin DC, we used mAb to two C-type lectin endocytic receptors, DEC-205/CD205 and langerin/CD207. When applied to murine and human skin explant cultures, these mAb were efficiently taken up by epidermal LC. Additionally, anti-DEC-205 targeted langerin+ CD103+ and langerin− CD103− mouse dermal DC. Unexpectedly, intradermal injection of either mAb, but not isotype control, resulted in strong and rapid labelling of LC in situ, implying that large molecules can diffuse through the basement membrane into the epidermis. Epidermal LC targeted in vivo by ovalbumin-coupled anti-DEC-205 potently presented antigen to CD4+ and CD8+ T cells. Thus, epidermal LC play a major role in uptake of lectin-binding ligands under standard vaccination conditions.
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影响因子：
32.4
作者：
Valladeau, J;Ravel, O;Saeland, S
通讯作者：
Saeland, S
DOI：
10.1084/jem.194.6.769
发表时间：
2001-09-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hawiger D;Inaba K;Dorsett Y;Guo M;Mahnke K;Rivera M;Ravetch JV;Steinman RM;Nussenzweig MC
通讯作者：
Nussenzweig MC
影响因子：
4.4
作者：
Carter, Robert W.;Thompson, Clare;Tough, David F.
通讯作者：
Tough, David F.
影响因子：
4.4
作者：
Stoitzner, Patrizia;Green, Laura K.;Ronchese, Franca
通讯作者：
Ronchese, Franca
影响因子：
15.3
作者：
Bonifaz, LC;Bonnyay, DP;Charalambous, A;Darguste, DI;Fujii, SI;Soares, H;Brimnes, MK;Moltedo, B;Moran, TM;Steinman, RM
通讯作者：
Steinman, RM