A Multi-Mineral Intervention to Modulate Colonic Mucosal Protein Profile: Results from a 90-Day Trial in Human Subjects.
A Multi-Mineral Intervention to Modulate Colonic Mucosal Protein Profile: Results from a 90-Day Trial in Human Subjects.
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调整结肠粘膜蛋白谱的多矿物干预：人类受试者90天试验的结果。
DOI：
10.3390/nu13030939
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发表时间：
2021-03-14
期刊：
影响因子：
5.9
通讯作者：
Varani J
中科院分区：
文献类型：
--
作者：
Aslam MN;McClintock SD;Jawad-Makki MAH;Knuver K;Ahmad HM;Basrur V;Bergin IL;Zick SM;Sen A;Turgeon DK;Varani J
这项研究的总体目标是确定富含钙、镁、微量元素的红藻衍生天然产品Aquamin®是否会改变结肠中参与生长调节和分化的蛋白质的表达。30名健康受试者(有患结直肠癌的风险)参加了为期90天的三臂介入试验。将Aquamin®与单独的钙剂和安慰剂进行比较。在介入期前后，取结肠活检。免疫组织化学检测Ki67(增殖标记物)和CK20、p21(分化标记物)的表达。基于串联质量标签-质谱仪的检测用于评估多个蛋白质的水平。与安慰剂或钙剂相比，Aquamin®降低了Ki67的表达水平，并略微增加了CK20的表达。钙离子和水通道蛋白®均能增加p21的表达。在蛋白质组筛查中，与安慰剂相比，Aquamin®治疗导致更多蛋白质上调(包括促凋亡、细胞角蛋白、细胞-细胞黏附分子和基底膜成分)或下调(增殖和核酸代谢)。单靠钙也可以改变许多相同蛋白质的表达，但程度不如Aquamin®。我们的结论是，每天摄入Aquamin®会改变结肠中的蛋白质表达谱，这可能有利于结肠健康。
The overall goal of this study was to determine whether Aquamin®, a calcium-, magnesium-, trace element-rich, red algae-derived natural product, would alter the expression of proteins involved in growth-regulation and differentiation in colon. Thirty healthy human subjects (at risk for colorectal cancer) were enrolled in a three-arm, 90-day interventional trial. Aquamin® was compared to calcium alone and placebo. Before and after the interventional period, colonic biopsies were obtained. Biopsies were evaluated by immunohistology for expression of Ki67 (proliferation marker) and for CK20 and p21 (differentiation markers). Tandem mass tag-mass spectrometry-based detection was used to assess levels of multiple proteins. As compared to placebo or calcium, Aquamin® reduced the level of Ki67 expression and slightly increased CK20 expression. Increased p21 expression was observed with both calcium and Aquamin®. In proteomic screen, Aquamin® treatment resulted in many more proteins being upregulated (including pro-apoptotic, cytokeratins, cell–cell adhesion molecules, and components of the basement membrane) or downregulated (proliferation and nucleic acid metabolism) than placebo. Calcium alone also altered the expression of many of the same proteins but not to the same extent as Aquamin®. We conclude that daily Aquamin® ingestion alters protein expression profile in the colon that could be beneficial to colonic health.
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DOI：
10.1007/s00198-017-4230-x
发表时间：
2017-12
期刊：
Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA
影响因子：
--
作者：
Balk EM;Adam GP;Langberg VN;Earley A;Clark P;Ebeling PR;Mithal A;Rizzoli R;Zerbini CAF;Pierroz DD;Dawson-Hughes B;International Osteoporosis Foundation Calcium Steering Committee
通讯作者：
International Osteoporosis Foundation Calcium Steering Committee
DOI：
10.1016/j.jsbmb.2015.01.010
发表时间：
2015-04
影响因子：
4.1
作者：
Bostick, Roberd M.
通讯作者：
Bostick, Roberd M.
影响因子：
3.9
作者：
Aslam, Muhammad Nadeem;Bergin, Ingrid;Varani, James
通讯作者：
Varani, James
DOI：
10.1016/j.bbamcr.2015.02.011
发表时间：
2015-09
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Aggarwal A;Prinz-Wohlgenannt M;Tennakoon S;Höbaus J;Boudot C;Mentaverri R;Brown EM;Baumgartner-Parzer S;Kállay E
通讯作者：
Kállay E
影响因子：
158.5
作者：
Baron, JA;Beach, M;Greenberg, ER
通讯作者：
Greenberg, ER