Psychological stress alters the ultrastructure and increases IL-1β and TNF-α in mandibular condylar cartilage.
Psychological stress alters the ultrastructure and increases IL-1β and TNF-α in mandibular condylar cartilage.
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DOI：
10.1590/s0100-879x2012007500102
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发表时间：
2012-10
期刊：
影响因子：
--
通讯作者：
Chen YJ
中科院分区：
文献类型：
--
作者：
Lv X;Li Q;Wu S;Sun J;Zhang M;Chen YJ
心理因素可以与颞下颌关节紊乱病(TMD)相关，但其机制尚不清楚。在本研究中，我们观察了在心理应激动物模型中，颞下颌关节(TMJ)髁状突软骨的超微结构变化和促炎细胞因子的表达。雄性SD大鼠(8周龄，2 10±10 g)，随机分为3组：心理应激组(PS，N=48)、足休克组(FS，N=2 4)和对照组(N=48)。在与FS大鼠建立心理应激1周、3周和5周后，处死PS大鼠，并与与PS大鼠相同时间处死的无应激对照组仔鼠进行比较。测量体重和肾上腺重量，用放射免疫法测定皮质酮和促肾上腺皮质激素水平。苏木精-伊红染色后进行组织学观察，扫描电子显微镜观察TMJ的超微结构。用双抗体夹心酶联免疫吸附试验和半定量逆转录聚合酶链式反应检测白细胞介素1β(IL 1β)和肿瘤坏死因子α(肿瘤坏死因子α)的转录和蛋白水平。PS组大鼠在应激3周后肾上腺重量显著增加，1、3、5周时激素水平明显升高，3、5周时出现明显的组织病理学改变和软骨变薄。PS组在应激1、3、5周时肿瘤坏死因子-α升高，IL-1β显著升高，5周后降至正常水平。心理应激使血浆激素水平升高，逆转录-聚合酶链式反应显示关节内IL-1β和肿瘤坏死因子-α的表达呈时间依赖性增加。这些结果表明，细胞因子的上调伴随着应力诱导的下颌骨软骨退变。促炎细胞因子在启动软骨破坏中发挥潜在作用，最终导致TMDS。
Psychological factors can be correlated with temporomandibular disorders (TMDs), but the mechanisms are unknown. In the present study, we examined the microstructural changes and expression of proinflammatory cytokines in mandibular condylar cartilage of the temporomandibular joint (TMJ) in a psychological stress animal model. Male Sprague-Dawley rats (8 weeks old, 210 ± 10 g) were randomly divided into 3 groups: psychological stress (PS, N = 48), foot shock (FS, N = 24), and control (N = 48). After inducing psychological stress using a communication box with the FS rats for 1, 3, or 5 weeks, PS rats were sacrificed and compared to their matched control littermates, which received no stress and were killed at the same times as the PS rats. Body and adrenal gland weight were measured and corticosterone and adrenocorticotropic hormone levels were determined by radioimmunoassay. After hematoxylin-eosin staining for histological observation, the ultrastructure of the TMJ was examined by scanning electron microscopy. Transcription and protein levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were evaluated by ELISA and semi-quantitative RT-PCR. The PS group showed a significantly higher adrenal gland weight after 3 weeks of stress and higher hormone levels at weeks 1, 3, and 5. Histopathological changes and thinning cartilage were apparent at weeks 3 and 5. In the PS group, TNF-α increased at 1, 3, and 5 weeks and IL-1β increased significantly after 1 and 3 weeks of stress, and then decreased to normal levels by 5 weeks. Psychological stress increased plasma hormone levels and RT-PCR indicated increased IL-1β and TNF-α expression in the TMJ in a time-dependent manner. These results suggest that cytokine up-regulation was accompanied by stress-induced cartilage degeneration in the mandibular condyle. The proinflammatory cytokines play a potential role in initiating the cartilage destruction that eventually leads to the TMDs.
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DOI：
10.14219/jada.archive.1990.0049
发表时间：
1990-03-01
影响因子：
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