Differential accumulation of phenolic compounds and expression of related genes in black- and yellow-seeded Brassica napus.
Differential accumulation of phenolic compounds and expression of related genes in black- and yellow-seeded Brassica napus.
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黑籽和黄籽甘蓝型油菜酚类化合物积累差异及相关基因表达
DOI：
10.1093/jxb/ert148
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发表时间：
2013-07
影响因子：
6.9
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Qu C;Fu F;Lu K;Zhang K;Wang R;Xu X;Wang M;Lu J;Wan H;Zhanglin T;Li J
开发具有改良品质的黄籽甘蓝型油菜（油菜籽）是主要育种目标。苯丙素和类黄酮途径的中间和最终代谢产物不仅影响油的质量，而且影响B的种皮颜色。油菜。本文分析了黑籽（ZY 821）和黄籽（GH 06）B种皮中酚类化合物的积累。油菜。采用甲苯胺蓝O染色和液相色谱-质谱联用技术，对ZY 821和GH 06在酚类物质积累方面的组织化学和生化差异进行了鉴定。在ZY 821和GH 06中分别检测到2个和13个黄酮醇衍生物。实时荧光定量PCR分析表明，ZY 821和GH 06在苯丙烷生物合成基因的表达上存在显著差异（BnPAL和BnC 4 H），共同的类黄酮生物合成基因（BnTT 4和BnTT 6），花青素和原花青素特异性基因（BnTT 3和BnTT 18），原花青素特异性基因（BnTT 12、BnTT 10和BnUGT 2）和在类黄酮生物合成途径中起作用的三种转录因子基因（BnTTG 1、BnTTG 2和BnTT 8）。这些数据提供了对B中色素积累的深入了解。为研究人员分析B中种皮颜色的形成和潜在的调控机制提供了有用的资源。油菜。
Developing yellow-seeded Brassica napus (rapeseed) with improved qualities is a major breeding goal. The intermediate and final metabolites of the phenylpropanoid and flavonoid pathways affect not only oil quality but also seed coat colour of B. napus. Here, the accumulation of phenolic compounds was analysed in the seed coats of black-seeded (ZY821) and yellow-seeded (GH06) B. napus. Using toluidine blue O staining and liquid chromatography–mass spectrometry, histochemical and biochemical differences were identified in the accumulation of phenolic compounds between ZY821 and GH06. Two and 13 unique flavonol derivatives were detected in ZY821 and GH06, respectively. Quantitative real-time PCR analysis revealed significant differences between ZY821 and GH06 in the expression of common phenylpropanoid biosynthetic genes (BnPAL and BnC4H), common flavonoid biosynthetic genes (BnTT4 and BnTT6), anthocyanin- and proanthocyandin-specific genes (BnTT3 and BnTT18), proanthocyandin-specific genes (BnTT12, BnTT10, and BnUGT2) and three transcription factor genes (BnTTG1, BnTTG2, and BnTT8) that function in the flavonoid biosynthetic pathway. These data provide insight into pigment accumulation in B. napus, and serve as a useful resource for researchers analysing the formation of seed coat colour and the underlying regulatory mechanisms in B. napus.
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影响因子：
7.4
作者：
Blount, JW;Korth, KL;Dixon, RA
通讯作者：
Dixon, RA
影响因子：
6.1
作者：
Lin LZ;Harnly JM
通讯作者：
Harnly JM
影响因子：
4.3
作者：
Auger B;Baron C;Lucas MO;Vautrin S;Bergès H;Chalhoub B;Fautrel A;Renard M;Nesi N
通讯作者：
Nesi N
影响因子：
4.3
作者：
Liang, Mingxiang;Davis, Elizabeth;Wu, Yajun
通讯作者：
Wu, Yajun
来自甘蓝型油菜和亲本物种的透明测试 12 基因：克隆、进化和黄色种子性状的差异参与
影响因子：
3.1
作者：
Chai, You-Rong;Lei, Bo;Chen, Li
通讯作者：
Chen, Li