Brassica orthologs from BANYULS belong to a small multigene family, which is involved in procyanidin accumulation in the seed.
Brassica orthologs from BANYULS belong to a small multigene family, which is involved in procyanidin accumulation in the seed.
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DOI：
10.1007/s00425-009-1017-0
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发表时间：
2009-11
期刊：
影响因子：
4.3
通讯作者：
Nesi N
中科院分区：
文献类型：
--
作者：
Auger B;Baron C;Lucas MO;Vautrin S;Bergès H;Chalhoub B;Fautrel A;Renard M;Nesi N
作为甘蓝型油菜种皮类黄酮生物合成研究的一部分。（油菜）中，将编码花色素还原酶的BANYULS基因的直向同源物克隆到B中。napus以及相关物种Brassica rapa和Brassica oleracea中。B。napus基因组含有四个BAN的功能拷贝，每个二倍体祖先有两个。氨基酸序列在包括B在内的菊科植物中高度保守。napus，B. rapa，B.甘蓝以及模式植物拟南芥。Bna.BAN启动子（ProBna.BAN）在ATG密码子5′端的沿着200 bp处与AtANR启动子保守，含有推测的顺式作用元件。此外，产生了携带与UidA报告基因融合的ProBna.BAN的前230 bp的转基因拟南芥和油菜植物。在两种菊科背景中，ProBna.BAN活性仅限于种皮。在B。BAN在内珠被最内层和珠孔合点区的原花青素积累细胞中被激活。在转录水平上，Bna、BAN基因在种子中均有表达。种子珠被的激光显微切割分析表明，Bna.BAN的表达仅限于内珠被，这与ProBna. BAN的激活模式一致。最后，将Bna、BAN基因定位到油菜遗传图谱上，并讨论了与粒色数量性状基因座的共定位。
As part of a research programme focused on flavonoid biosynthesis in the seed coat of Brassica napus L. (oilseed rape), orthologs of the BANYULS gene that encoded anthocyanidin reductase were cloned in B. napus as well as in the related species Brassica rapa and Brassica oleracea. B. napus genome contained four functional copies of BAN, two originating from each diploid progenitor. Amino acid sequences were highly conserved between the Brassicaceae including B. napus, B. rapa, B. oleracea as well as the model plant Arabidopsis thaliana. Along the 200 bp in 5′ of the ATG codon, Bna.BAN promoters (ProBna.BAN) were conserved with AtANR promoter and contained putative cis-acting elements. In addition, transgenic Arabidopsis and oilseed rape plants carrying the first 230 bp of ProBna.BAN fused to the UidA reporter gene were generated. In the two Brassicaceae backgrounds, ProBna.BAN activity was restricted to the seed coat. In B. napus seed, ProBna.BAN was activated in procyanidin-accumulating cells, namely the innermost layer of the inner integument and the micropyle-chalaza area. At the transcriptional level, the four Bna.BAN genes were expressed in the seed. Laser microdissection assays of the seed integuments showed that Bna.BAN expression was restricted to the inner integument, which was consistent with the activation profile of ProBna.BAN. Finally, Bna.BAN genes were mapped onto oilseed rape genetic maps and potential co-localisations with seed colour quantitative trait loci are discussed.
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DOI：
10.1073/pnas.0510791103
发表时间：
2006-03-28
影响因子：
11.1
作者：
Lysak, MA;Berr, A;Schubert, I
通讯作者：
Schubert, I
来自甘蓝型油菜和亲本物种的透明测试 12 基因：克隆、进化和黄色种子性状的差异参与
影响因子：
3.1
作者：
Chai, You-Rong;Lei, Bo;Chen, Li
通讯作者：
Chen, Li
影响因子：
7.2
作者：
Devic, M;Guilleminot, J;Delseny, M
通讯作者：
Delseny, M
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
7
作者：
Lysak, MA;Koch, MA;Schubert, I
通讯作者：
Schubert, I