Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies.
Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies.
复制标题
DOI：
10.3390/vetsci5010012
复制
发表时间：
2018-01-22
影响因子：
2.4
通讯作者：
Oura C
中科院分区：
文献类型：
--
作者：
Brown Jordan A;Blake D;Beard J;Beharry A;Serrette L;Soleyn A;Sookhoo J;Blake L;Brown G;Oura C
球虫病是一种危害鸡的肠道疾病，对世界范围内的肉鸡生产具有重要的经济意义。在鸡中发现的球虫物种包括堆型艾美耳球虫、布氏艾美耳球虫、巨型艾美耳球虫、缓型艾美耳球虫、毒害艾美耳球虫、早熟艾美耳球虫和柔嫩艾美耳球虫。近年来，聚合酶链反应（PCR）已发展到提供准确和快速的鉴定七种已知的鸡艾美耳球虫。本研究的目的是使用物种特异性实时PCR（qPCR），以确定这七个艾美耳球虫物种存在于特立尼达家禽。从特立尼达岛的6个肉鸡养殖场（每个养殖场2 - 5个围栏）收集了17份合并粪便样品。还从两个活禽市场（拔毛店）中显示球虫病临床体征的禽类采集粪便。qPCR显示存在5种艾美耳球虫（E. acervulina、E. maxima，E. mitis、细叶假单胞菌E. necatrix和E. tenella），而不是E. brunetti或E.早熟结果表明，所有肉鸡场均检测到混合感染，并检测到两种高致病性艾美耳球虫（E。tenella和E.从两间拔毛店抽取的临床病禽粪便样本中检测到necatrix）。
Coccidiosis is an intestinal disease of chickens of major economic importance to broiler industries worldwide. Species of coccidia found in chickens include Eimeria acervulina, Eimeria brunetti, Eimeria maxima, Eimeria mitis, Eimeria necatrix, Eimeria praecox, and Eimeria tenella. In recent years, polymerase chain reaction (PCR) has been developed to provide accurate and rapid identification of the seven known Eimeria species of chickens. The aim of this study was to use species-specific real-time PCR (qPCR) to identify which of the seven Eimeria species are present in Trinidad poultry. Seventeen pooled fecal samples were collected from 6 broiler farms (2–5 pens per farm) across Trinidad. Feces were also collected from birds showing clinical signs of coccidiosis in two live bird markets (pluck shops). qPCR revealed the presence of five species of Eimeria (E. acervulina, E. maxima, E. mitis, E. necatrix, and E. tenella), but not E. brunetti or E. praecox. Mixed infections were detected on all broiler farms, and DNA of two highly pathogenic Eimeria species (E. tenella and E. necatrix) was detected in feces taken from clinically sick birds sampled from the two pluck shops.
登录
查看更多内容
影响因子：
2.8
作者：
Haug, Anita;Gjevre, Anne-Gerd;Kaldhusdal, Magne
通讯作者：
Kaldhusdal, Magne
DOI：
10.1111/j.1550-7408.1984.tb05498.x
发表时间：
1984-01-01
期刊：
JOURNAL OF PROTOZOOLOGY
影响因子：
--
作者：
LONG, PL;JOYNER, LP
通讯作者：
JOYNER, LP
影响因子：
4
作者：
Williams, RB
通讯作者：
Williams, RB
家用鸡的球虫病的致病药物的基因组分析。
影响因子：
7
作者：
Reid AJ;Blake DP;Ansari HR;Billington K;Browne HP;Bryant J;Dunn M;Hung SS;Kawahara F;Miranda-Saavedra D;Malas TB;Mourier T;Naghra H;Nair M;Otto TD;Rawlings ND;Rivailler P;Sanchez-Flores A;Sanders M;Subramaniam C;Tay YL;Woo Y;Wu X;Barrell B;Dear PH;Doerig C;Gruber A;Ivens AC;Parkinson J;Rajandream MA;Shirley MW;Wan KL;Berriman M;Tomley FM;Pain A
通讯作者：
Pain A
影响因子：
2.6
作者：
Carvalho, Fabio Santos;Wenceslau, Amauri Arias;Albuquerque, George Rego
通讯作者：
Albuquerque, George Rego