Multiplexed determination of intracellular messenger RNA by using a graphene oxide nanoprobe modified with target-recognizing fluorescent oligonucleotides
Multiplexed determination of intracellular messenger RNA by using a graphene oxide nanoprobe modified with target-recognizing fluorescent oligonucleotides
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使用经过目标识别荧光寡核苷酸修饰的氧化石墨烯纳米探针对细胞内信使 RNA 进行多重测定。
DOI：
10.1007/s00604-018-3090-1
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发表时间：
2018-11
影响因子：
5.7
通讯作者：
Ling Kai
中科院分区：
文献类型：
--
作者：
Jiang Hongyan;Li Fu-Rong;Li Wei;Lu Xiaodong;Ling Kai
描述了用于活细胞中信使RNA（mRNA）的定量和成像的多重氧化石墨烯（GO）荧光纳米探针。用不同的荧光染料标记识别寡核苷酸（具有与靶mRNA的序列互补的序列）。如果吸附在GO上，则识别寡核苷酸的荧光被淬灭。在穿透活细胞后，寡核苷酸与靶mRNA结合并从GO解离。这导致荧光的恢复。使用不同的荧光染料，可以在短时间内通过高含量分析同时对各种细胞内mRNA进行成像和定量。肌动蛋白mRNA作为内部对照。这种基于GO的纳米探针允许在1至400 nM的分析范围内测定mRNA模拟物，检测限低至0.26 nM。在单个活细胞中可以同时检测多达3种细胞内mRNA（C-myc、TK 1和肌动蛋白）。因此，这种纳米探针能够特异性区分癌细胞和正常细胞中的细胞内mRNA表达水平。它可以潜在地应用作为一种工具，用于检测癌症的进展和diagnosis.Graphical abstractA复用氧化石墨烯（GO）为基础的荧光纳米探针被描述为定量和成像的细胞内信使RNA。在穿透活细胞后，可以分析解离的识别寡核苷酸的回收荧光，这允许同时检测多达3种细胞内信使RNA。
A multiplexed graphene oxide (GO) fluorescent nanoprobe is described for quantification and imaging of messenger RNAs (mRNAs) in living cells. The recognizing oligonucleotides (with sequences complementary to those of target mRNAs) were labeled with different fluorescent dyes. If adsorbed on GO, the fluorescence of the recognizing oligonucleotides is quenched. After having penetrated living cells, the oligonucleotides bind to target mRNAs and dissociate from GO. This leads to the recovery of fluorescence. Using different fluorescent dyes, various intracellular mRNAs can be simultaneously imaged and quantified by a high content analysis within a short period of time. Actin mRNA acts as the internal control. This GO-based nanoprobe allows mRNA mimics to be determined within an analytical range from 1 to 400 nM and a detection limit as low as 0.26 nM. Up to 3 intracellular mRNAs (C-myc, TK1, and actin) can be detected simultaneously in a single living cell. Hence, this nanoprobe enables specific distinction of intracellular mRNA expression levels in cancerous and normal cells. It can be potentially applied as a tool for detection of cancer progression and diagnosis.Graphical abstractA multiplexed graphene oxide (GO)-based fluorescent nanoprobe is described for quantification and imaging of intracellular messenger RNAs. After penetrating living cells, the recovered fluorescence of the dissociated recognizing oligonucleotides can be analyzed , and this allows for simultaneous detection of up to 3 intracellular messenger RNAs.
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DOI：
10.1002/smll.201401137
发表时间：
2015-05
期刊：
Small (Weinheim an der Bergstrasse, Germany)
影响因子：
--
作者：
Giraldo-Vela JP;Kang W;McNaughton RL;Zhang X;Wile BM;Tsourkas A;Bao G;Espinosa HD
通讯作者：
Espinosa HD
影响因子：
10.8
作者：
Zheng D;Seferos DS;Giljohann DA;Patel PC;Mirkin CA
通讯作者：
Mirkin CA
影响因子：
3.5
作者：
Chen, Chung-Chi;Chang, Tsai-Wang;Lin, Shiu-Ru
通讯作者：
Lin, Shiu-Ru
影响因子：
6.4
作者：
Morris S;Vachani A;Pass HI;Rom WN;Ryden K;Weiss GJ;Hogarth DK;Runger G;Richards D;Shelton T;Mallery DW
通讯作者：
Mallery DW
影响因子：
--
作者：
Sreejith Mangalath;Silja Abraham;Joshy Joseph
通讯作者：
Sreejith Mangalath;Silja Abraham;Joshy Joseph