RNA fingerprinting by arbitrarily primed PCR.
RNA fingerprinting by arbitrarily primed PCR.
复制标题
通过任意引物 PCR 进行 RNA 指纹分析。
DOI:
10.1101/gr.4.1.s66
复制
发表时间:
1994
期刊:
影响因子:
--
通讯作者:
Welsh,J
中科院分区:
文献类型:
--
作者:
McClelland,M;Welsh,J
One of the great strengths of the family of arbitrarily primed PCR methods is their simplicity. 1'2) The extension of arbitrarily primed PCR fingerprinting to RNA (3'4) has resulted in a tool with exciting potential for detecting differential gene expression. It is now possible to obtain a partly abundance-normalized sample of cDNAs produced in a single tube in a few hours. Fragments of differentially expressed genes can be cloned directly from PCR-amplified products isolated from the gel.The method can provide a complex phenotype reflecting changes in the abundance of hundreds of RNAs under various conditions. Comparison of RNA fingerprints from different treatment groups allows one to draw inferences regarding gene regulation. Hypotheses regarding signal transduction can be tested and new hypotheses generated using this information. In this brief methods-oriented review we will justify our current preferred protocol for RNA fingerprinting. An accompanying article will discuss DNA fingerprinting.(s)
登录
查看更多内容
影响因子:
14.9
作者:
McClelland,M;Arensdorf,H;Cheng,R;Welsh,J
通讯作者:
Welsh,J
影响因子:
4.6
作者:
David E. Smith;T. Gridley
通讯作者:
T. Gridley
DOI:
--
发表时间:
1993
期刊:
影响因子:
--
作者:
K. Yu;A. Deynze;K. P. Pauls;B. Glick;J. E. Thompson
通讯作者:
J. E. Thompson
DOI:
--
发表时间:
1973
期刊:
The Quarterly review of biology
影响因子:
--
作者:
E. Davidson;R. Britten
通讯作者:
R. Britten
影响因子:
5.2
作者:
K. Wong;C. Mok;J. Welsh;Michael McClelland;S. Tsao;R. Berkowitz
通讯作者:
R. Berkowitz