Differential screening of a PCR-generated mouse embryo cDNA library: glucose transporters are differentially expressed in early postimplantation mouse embryos.
Differential screening of a PCR-generated mouse embryo cDNA library: glucose transporters are differentially expressed in early postimplantation mouse embryos.
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PCR 生成的小鼠胚胎 cDNA 文库的差异筛选：葡萄糖转运蛋白在植入后早期小鼠胚胎中差异表达。
作者：
David E. Smith;T. Gridley
Differential screening of a cDNA library constructed using PCR amplification techniques from RNA isolated from the distal portion (embryonic ectoderm, mesoderm and visceral endoderm) of 7.5 days post coitum (dpc) mouse embryos led to the isolation of two cDNA clones expressed at higher levels in 7.5 dpc embryos than 12.5 dpc embryos. Nucleotide sequence analysis revealed that each of these clones was a different member of the family of facilitative glucose transporters (Glut genes). The differentially expressed cDNA clones represent mouse Glut-1 and Glut-3. Levels of the Glut-3 mRNA declined 14-fold between days 7.5 and 12.5 of gestation, and were under our limits of detection by 14.5 dpc. The levels of the Glut-1 mRNA declined about 3-fold between days 7.5 and 12.5 of gestation. Analysis of the expression of these genes by in situ hybridization revealed striking differences in transcript localization in early postimplantation mouse embryos. At 7.5 dpc, both transporters were expressed more strongly in extraembryonic tissues than in the embryo proper. While both transporters were expressed in the amnion and chorion, only Glut-1 was expressed in the ectoplacental cone. In the yolk sac, Glut-3 appeared to be expressed only in the endoderm while Glut-1, although expressed in both layers, was expressed more strongly in the mesoderm layer. Thus, the two transporters have relatively reciprocal sites of expression in the developing extraembryonic membranes. Expression of Glut-1 was fairly widespread in the embryo at 8.5 dpc, but by 10.5 dpc expression was down-regulated and was observed in the eye and the spinal cord.(ABSTRACT TRUNCATED AT 250 WORDS)
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小鼠植入前发育过程中葡萄糖转运蛋白分子的发育表达和细胞定位。
DOI：
10.1242/dev.115.1.305
发表时间：
1992
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Aghayan,M;Rao,LV;Smith,RM;Jarett,L;Charron,MJ;Thorens,B;Heyner,S
通讯作者：
Heyner,S
影响因子：
18.2
作者：
J. Pessin;G. Bell
通讯作者：
J. Pessin;G. Bell
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Nagamatsu,S;Kornhauser,JM;Burant,CF;Seino,S;Mayo,KE;Bell,GI
通讯作者：
Bell,GI
母亲糖尿病对早期胚胎发生的影响：II。
DOI：
10.1002/tera.1420210311
发表时间：
1980
期刊：
Teratology
影响因子：
--
作者：
Sadler,TW
通讯作者：
Sadler,TW
PCR 扩增总 cDNA 的克隆：小鼠卵母细胞 cDNA 文库的构建。
DOI：
10.1016/0735-0651(90)90038-h
发表时间：
1990
期刊：
Genetic analysis, techniques and applications
影响因子：
--
作者：
Welsh,J;Liu,JP;Efstratiadis,A
通讯作者：
Efstratiadis,A