Expression of reverse cholesterol transport proteins ATP-binding cassette A1 (ABCA1) and scavenger receptor BI (SR-BI) in the retina and retinal pigment epithelium.
Expression of reverse cholesterol transport proteins ATP-binding cassette A1 (ABCA1) and scavenger receptor BI (SR-BI) in the retina and retinal pigment epithelium.
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DOI：
10.1136/bjo.2008.144006
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发表时间：
2009-08
期刊：
影响因子：
--
通讯作者：
Duncan JL
中科院分区：
文献类型：
--
作者：
Duncan KG;Hosseini K;Bailey KR;Yang H;Lowe RJ;Matthes MT;Kane JP;LaVail MM;Schwartz DM;Duncan JL
布鲁赫膜（Bruch’s membrane，BrM）中的过度脂质积累是衰老的标志，是年龄相关性黄斑变性（age-related macular degeneration，AMD）的主要危险因素。视网膜色素上皮（RPE）细胞可以利用胆固醇逆向转运（RCT）活性将脂质移动到BrM中，通过ATP结合盒A1（ABCA 1）和清道夫受体BI（SR-BI）介导。ABCA 1的表达通过逆转录聚合酶链反应（RT-PCR）和人RPE细胞提取物的蛋白质印迹法进行评估。使用放射性标记的光感受器外节（POS）进行脂质转运测定。免疫荧光染色检测正常小鼠眼内ABCA 1和SR-BI的表达。用显微镜检查ABCA 1和SR-BI杂合子小鼠的BrM。人RPE细胞表达ABCA 1 mRNA和蛋白。ABCA 1和SR-BI抑制剂格列本脲（也称为格列本脲）在高密度脂蛋白存在下废除RPE细胞中POS衍生脂质的基础转运。小鼠视网膜和RPE表达ABCA 1和SR-BI。SR-BI在RPE中高表达。BRMs在SR-BI杂合子小鼠中显著增厚，但在ABCA 1杂合子小鼠中没有。RPE细胞表达ABCA 1和SR-BI。这意味着SR-BI和ABCA 1在视网膜和RPE中的脂质转运和RCT中的重要作用。
Excessive lipid accumulation in Bruch’s membrane (BrM) is a hallmark of ageing, the major risk factor for age-related macular degeneration (AMD). Retinal pigment epithelial (RPE) cells may utilise reverse cholesterol transport (RCT) activity to move lipid into BrM, mediated through ATP-binding cassette A1 (ABCA1) and scavenger receptor BI (SR-BI). ABCA1 expression was assessed by reverse transcription polymerase chain reaction (RT-PCR) and western blotting of human RPE cell extracts. Lipid transport assays were performed using radiolabelled photoreceptor outer segments (POS). ABCA1 and SR-BI expression was examined in normal mouse eyes by immunofluorescence staining. BrMs of ABCA1 and SR-BI heterozygous mice were examined microscopically. Human RPE cells expressed ABCA1 mRNA and protein. The ABCA1 and SR-BI inhibitor glyburide (also known as glibenclamide) abolished basal transport of POS-derived lipids in RPE cells in the presence of high-density lipoprotein. Mouse retina and RPE expressed ABCA1 and SR-BI. SR-BI was highly expressed in RPE. BrMs were significantly thickened in SR-BI heterozygous mice, but not in ABCA1 heterozygous mice. RPE cells express ABCA1 and SR-BI. This implies a significant role for SR-BI and ABCA1 in lipid transport and RCT in the retina and RPE.
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影响因子：
3.4
作者：
Bok, D;Yasumura, D;Lavail, MM
通讯作者：
Lavail, MM
影响因子：
--
作者：
Dithmar, S;Sharara, NA;Grossniklaus, HE
通讯作者：
Grossniklaus, HE
DOI：
10.1006/bbrc.2002.6756
发表时间：
2002-04-12
影响因子：
3.1
作者：
Duncan, KG;Bailey, KR;Schwartz, DM
通讯作者：
Schwartz, DM
影响因子：
30.8
作者：
Bodzioch, M;Orsó, E;Schmitz, G
通讯作者：
Schmitz, G
影响因子：
6.5
作者：
Nieland, TJF;Chroni, A;Krieger, M
通讯作者：
Krieger, M