Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism.
Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism.
复制标题
DOI：
10.3390/microorganisms10030505
复制
发表时间：
2022-02-25
期刊：
影响因子：
4.5
通讯作者：
Laouini D
中科院分区：
文献类型：
--
作者：
Attia H;Sghaier MR;Bali A;Guerfali FZ;Chlif S;Atri C;Belhaj-Hamida N;Zaatour A;Gharbi A;Ben-Salah A;Dellagi K;Laouini D
The clinical expression of zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania (L.) major parasites has a broad spectrum ranging from asymptomatic infection to self-limited cutaneous sores or severe disease. In concert with the host immune responses, the vector variability and the number of bites, genetic variation between L. major isolates might impact on the clinical output of the disease. We investigated herein the intra-specific variability of L. major field isolates independently of host or vector factors and then tried to correlate parasite variability to ZCL severity in corresponding patients. Several assays were applied, i.e., in vivo pathogenicity of promastigotes in a BALB/c mice model, resistance/sensibility to complement lysis, in vitro growth kinetics, and expression of different lectins on the promastigote surface. Combining all these parameters allowed us to conclude that the resistance to complement lysis and PNA/Jacalin lectins binding to parasite surfaces are important markers of parasite virulence. These factors correlate significantly with clinic polymorphism of ZCL and modestly with genetic micro-heterogeneity, a characteristic we previously revealed with a MLMT profile.
登录
查看更多内容
影响因子：
5.7
作者：
Forestier, Claire-Lise;Gao, Qi;Boons, Geert-Jan
通讯作者：
Boons, Geert-Jan
影响因子：
2
作者：
Andrade, AFB;Saraiva, FMB
通讯作者：
Saraiva, FMB
影响因子：
2.7
作者：
Noronha, FSM;Nunes, AC;Ramalho-Pinto, FJ
通讯作者：
Ramalho-Pinto, FJ
影响因子：
1.9
作者：
Ramos, Patricia K. S.;Diniz, Jose A. P.;de Souza, Wanderley
通讯作者：
de Souza, Wanderley
影响因子：
2.1
作者：
HOWARD, MK;SAYERS, G;MILES, MA
通讯作者：
MILES, MA