Nylon-3 copolymers that generate cell-adhesive surfaces identified by library screening.
Nylon-3 copolymers that generate cell-adhesive surfaces identified by library screening.
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DOI：
10.1021/ja9050636
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发表时间：
2009-11-25
影响因子：
15
通讯作者：
Masters, Kristyn S.
中科院分区：
文献类型：
--
作者：
Lee, Myung-Ryul;Stahl, Shannon S.;Gellman, Samuel H.;Masters, Kristyn S.
尼龙-3家族中的聚合物含有衍生自β-氨基酸的亚基，其通过酰胺键彼此连接。因此，尼龙-3骨架与蛋白质的α-氨基酸骨架同源。这种分子水平的同源性表明，尼龙-3材料可能本质上是蛋白质模拟物。这里描述的实验探索这种前景的背景下，细胞粘附，组织工程作为一个长期的目标。我们评估了一个小型序列随机尼龙-3共聚物库，以评估其使表面对NIH 3 T3成纤维细胞粘附和扩散有吸引力的能力。通过将二维阵列中的共聚物附着到改性的玻璃表面，以高通量、平行模式完成文库筛选。观察到成纤维细胞粘附和扩散的显着变化作为尼龙-3亚基的身份和比例的函数。几种尼龙-3共聚物支持的细胞粘附和形态与阳性对照基质（如组织培养聚苯乙烯和胶原蛋白涂层玻璃）相当，甚至上级。此外，在无血清条件下进行的研究表明，特定的尼龙-3衍生物支持细胞粘附独立于血清蛋白吸附。尽管在不存在血清的情况下细胞粘附减少，但特定共聚物显示出支持比任何其他条件（包括阳性对照）显著更大的细胞粘附的能力。尼龙-3共聚物，这是最有效的促进粘附到改性的玻璃表面证明也是有效的，当连接到PEG基水凝胶，促进粘附，证明了这些共聚物的潜力，用于组织工程应用。
Polymers in the nylon-3 family contain subunits derived from β-amino acids, which are linked to one another via amide bonds. Thus, the nylon-3 backbone is homologous to the α-amino acid-based backbone of proteins. This molecular-level homology suggests that nylon-3 materials might be intrinsically protein-mimetic. The experiments described here explore this prospect in the context of cell adhesion, with tissue engineering as a long-range goal. We have evaluated a small library of sequence-random nylon-3 copolymers for the ability to render surfaces attractive to NIH 3T3 fibroblast adhesion and spreading. Library screening was accomplished in a high-throughput, parallel mode via attachment of the copolymers in a two-dimensional array to a modified glass surface. Significant variations in fibroblast adhesion and spreading were observed as a function of nylon-3 subunit identity and proportion. Several of the nylon-3 copolymers supported cell adhesion and morphology that was comparable, or even superior, to that achieved on positive control substrates such as tissue culture polystyrene and collagen-coated glass. Moreover, studies conducted under serum-free conditions demonstrated that specific nylon-3 derivatives supported cell adhesion independently of serum protein adsorption. Although cell adhesion was diminished in the absence of serum, particular copolymers demonstrated an ability to support substantially greater cell adhesion than any of the other conditions, including the positive controls. The nylon-3 copolymers that were most effective at promoting adhesion to a modified glass surface proved also to be effective at promoting adhesion when attached to a PEG-based hydrogel, demonstrating the potential for these copolymers to be used in tissue engineering applications.
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影响因子：
--
作者：
Gonzalez, AL;Gobin, AS;Smith, CW
通讯作者：
Smith, CW
影响因子：
56.9
作者：
RUOSLAHTI, E;PIERSCHBACHER, MD
通讯作者：
PIERSCHBACHER, MD
影响因子：
14
作者：
Nguyen, KT;West, JL
通讯作者：
West, JL
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14
作者：
Kretsinger, JK;Haines, LA;Schneider, JP
通讯作者：
Schneider, JP
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作者：
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通讯作者：
FELD, MK