Use of a two-stage insulin infusion study to assess the relationship between insulin suppression of lipolysis and insulin-mediated glucose uptake in overweight/obese, nondiabetic women.
Use of a two-stage insulin infusion study to assess the relationship between insulin suppression of lipolysis and insulin-mediated glucose uptake in overweight/obese, nondiabetic women.
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使用两阶段胰岛素输注研究来评估超重/肥胖、非糖尿病女性的胰岛素抑制脂肪分解与胰岛素介导的葡萄糖摄取之间的关系。
DOI：
10.1016/j.metabol.2011.05.008
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Reaven,Gerald
中科院分区：
文献类型：
--
作者：
McLaughlin,Tracey;Yee,Gail;Glassford,Alec;Lamendola,Cindy;Reaven,Gerald
在用于区分胰岛素介导的相对葡萄糖处理的相同胰岛素浓度下，游离脂肪酸（FFAs）的胰岛素调节差异并不明显。对胰岛素介导的葡萄糖处理的抵抗和全天较高的FFA浓度在肥胖个体中更常见。然而，在这一人群中，胰岛素抑制脂肪组织游离脂肪酸释放和刺激肌肉中葡萄糖处理的能力之间的关系尚未明确。目前的研究是为了验证胰岛素作用的这两个方面是相关的假设，即胰岛素介导的葡萄糖处置缺陷越大，胰岛素对脂肪组织释放游离脂肪酸的抑制效果越差。受试者包括56名健康的非糖尿病超重/中度肥胖妇女，根据全身葡萄糖处理分为胰岛素抵抗或胰岛素敏感。所有患者均接受改良的240分钟2期胰岛素输注，注射基础（~ 15 μ U/mL）和生理性升高（~ 80 μ U/mL）稳态胰岛素浓度。在整个过程中测量血浆葡萄糖、胰岛素、游离脂肪酸和甘油。胰岛素抵抗和胰岛素敏感受试者在生理性高胰岛素血症期间血浆葡萄糖差异最大，而血浆FFA/甘油在基础胰岛素浓度期间差异最大。基础胰岛素稳定状态下的FFA浓度与高胰岛素稳定状态下的葡萄糖浓度高度相关（r = 0.85, P < 0.001）。超重/中度肥胖的女性在胰岛素抑制血浆游离脂肪酸的能力上表现出显著的差异，这与胰岛素介导的葡萄糖处理的差异高度相关。胰岛素调节FFA的可变性在基础胰岛素浓度下最为明显，而葡萄糖处理的差异在生理性高胰岛素血症期间最为明显。两者都可以通过简单的两阶段胰岛素输注研究来量化，第一阶段FFA浓度和第二阶段葡萄糖浓度是最具信息量的。
Differences in insulin regulation of free fatty acids (FFAs) are not readily apparent at the same insulin concentrations used to differentiate relative insulin-mediated glucose disposal. Resistance to insulin-mediated glucose disposal and higher daylong FFA concentrations occur more commonly in obese individuals. However, the relationship between the ability of insulin to suppress FFA release from adipose tissue and stimulate glucose disposal in muscle has not been clearly defined in this population. The current study was initiated to test the hypothesis that these 2 facets of insulin action are related, with greater defects in insulin-mediated glucose disposal associated with less effective insulin inhibition of FFA release from adipose tissue. Subjects included 56 healthy nondiabetic overweight/moderately obese women classified as insulin resistant or insulin sensitive based on whole-body glucose disposal. All underwent a modified 240-minute 2-stage insulin infusion with basal (∼15 µU/mL) and physiologically elevated (∼80 µU/mL) steady-state insulin concentrations. Plasma glucose, insulin, FFA, and glycerol were measured throughout. Whereas plasma glucose differed most during physiological hyperinsulinemia in insulin-resistant vs insulin-sensitive subjects, plasma FFA/glycerol differed most during basal insulin concentrations. The FFA concentrations during the basal insulin steady state correlated highly (r = 0.85, P < .001) with glucose concentrations during the hyperinsulinemic steady state. Overweight/moderately obese women exhibit dramatic differences in the ability of insulin to suppress plasma FFA, which correlate highly with differences in insulin-mediated glucose disposal. Variability in insulin regulation of FFA is most apparent at basal insulin concentrations, whereas differences in glucose disposal are most apparent during physiologic hyperinsulinemia. Both can be quantified using a simple 2-stage insulin infusion study, with first-stage FFA concentrations and second-stage glucose concentrations being most informative.
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健康志愿者对生理性高胰岛素血症的葡萄糖处理与基础葡萄糖和游离脂肪酸浓度之间的关系。
DOI：
10.1210/jcem.85.3.6450
发表时间：
2000
期刊：
The Journal of clinical endocrinology and metabolism.
影响因子：
--
作者：
Abbasi,F;McLaughlin,T;Lamendola,C;Reaven,GM
通讯作者：
Reaven,GM
健康志愿者肌肉和脂肪组织脂肪分解胰岛素介导的葡萄糖处理之间的关系。
DOI：
10.1210/jcem.80.11.7593453
发表时间：
1995
期刊：
The Journal of clinical endocrinology and metabolism.
影响因子：
--
作者：
Pei,D;Chen,YD;Hollenbeck,CB;Bhargava,R;Reaven,GM
通讯作者：
Reaven,GM
影响因子：
15.9
作者：
SHEN, SW;REAVEN, GM;FARQUHAR, JW
通讯作者：
FARQUHAR, JW
影响因子：
9.8
作者：
HOWARD, BV;SAVAGE, PJ;BENNETT, PH
通讯作者：
BENNETT, PH
人类的葡萄糖代谢：对静脉注射葡萄糖的反应。
DOI：
10.1016/0026-0495(79)90066-0
发表时间：
1979
期刊：
Metabolism: clinical and experimental
影响因子：
--
作者：
Robert R. Wolfe;J. R. Allsop;John F. Burke
通讯作者：
John F. Burke