Enzyme-linked immunosorbent assay for the detection of antibodies to nutritionally variant streptococci in patients with endocarditis.

Enzyme-linked immunosorbent assay for the detection of antibodies to nutritionally variant streptococci in patients with endocarditis.
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用于检测心内膜炎患者营养变异链球菌抗体的酶联免疫吸附测定。

DOI:
10.1093/infdis/153.1.116
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发表时间:
1986
期刊:
The Journal of infectious diseases
影响因子:
--
通讯作者:
Roberts,RB
Roberts,RB
中科院分区:
--
文献类型:
--
作者:
vandeRijn,I;George,M;Bouvet,A;Roberts,RB

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草绿色链球菌是造成50%-55%的微生物性心内膜炎的原因。在这些不同的菌种中,营养变异链球菌(NVS)最近与培养阴性的心内膜炎有关,占所有链球菌心内膜炎的5%-10%。这些细菌的分离和鉴定受到其生长要求的极端苛刻性质以及其缓慢生长速率的阻碍。此外,他们的抗生素敏感性差异很大,这一特点导致发病率和死亡率高于非NVS心内膜炎患者。由于这些原因,采用酶联免疫吸附试验检测NVS心内膜炎患者血清中NVS血清型I抗原的抗体。74%的NVS心内膜炎患者对该抗原的滴度升高。对照组和非NVS链球菌性心内膜炎患者血清中均未出现滴度升高,仅6.7%的血清出现滴度升高。这些结果表明,NVS血清型I抗原的抗体可以作为NVS心内膜炎的标志物。
The viridans streptococci are responsible for 50%–55% of microbial endocarditis. Among these varied species, the nutritionally variant streptococci (NVS) have recently been associated with culture-negative endocarditis and are responsible for 5%–10% of all streptococcal endocarditis. The isolation and identification of these bacteria has been hampered by the extremely fastidious nature of their growth requirements as well as by their slow growth rate. In addition, their antibiotic sensitivity varies greatly, a characteristic leading to a higher rate of morbidity and mortality than is found in patients with non-NVS endocarditis. For these reasons sera from patients with NVS endocarditis were examined for antibodies to the NVS serotype I antigen by using an enzyme-linked immunosorbent assay. Seventy-four percent of patients with NVS endocarditis had elevated titers to this antigen. None of the sera from controls and only 6.7% of the sera from patients with non-NVS streptococcal endocarditis showed increased titers. These results indicate that antibody to the NVS serotype I antigen can serve as a marker for NVS endocarditis.
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