Mapping Bacterial Biofilm on Features of Orthopedic Implants In Vitro.
Mapping Bacterial Biofilm on Features of Orthopedic Implants In Vitro.
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DOI：
10.3390/microorganisms10030586
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发表时间：
2022-03-08
期刊：
影响因子：
4.5
通讯作者：
Stoodley P
中科院分区：
文献类型：
--
作者：
Moore K;Gupta N;Gupta TT;Patel K;Brooks JR;Sullivan A;Litsky AS;Stoodley P
植入物相关感染是骨科手术的主要并发症。假体周围关节感染中最常见的微生物之一是金黄色葡萄球菌，一种形成生物膜的病原体。骨科植入物由钛、聚乙烯和不锈钢等多种材料组成，这些材料存在细菌生物膜定殖的风险。关于整形外科硬件的较大表面特征（例如脊、孔、边缘等）如何影响生物膜的形成和附着，人们知之甚少。为了研究生物膜如何在实际部件上形成，我们将多个不同形状、尺寸、粗糙度和材料类型的骨科植入物浸入接种有金黄色葡萄球菌 SAP231（一种生物发光 USA300 菌株）的脑心输液肉汤中。将植入物孵育 72 小时，每天更换培养基。孵化后，使用体外成像系统（IVIS）对植入物进行成像，并通过图像分析对生物膜产生的代谢信号进行量化。然后使用扫描电子显微镜对植入物的不同区域进行成像，以补充 IVIS 成像。当图像合并时，与单个植入物上以及所有植入物上的边缘或光滑表面相比，粗糙表面具有最大的发光度。边缘的发光也明显大于光滑表面。这些数据表明，种植体的粗糙度以及大范围的表面特征可能面临更大的生物膜定植风险。
Implant-associated infection is a major complication of orthopedic surgery. One of the most common organisms identified in periprosthetic joint infections is Staphylococcus aureus, a biofilm-forming pathogen. Orthopedic implants are composed of a variety of materials, such as titanium, polyethylene and stainless steel, which are at risk for colonization by bacterial biofilms. Little is known about how larger surface features of orthopedic hardware (such as ridges, holes, edges, etc.) influence biofilm formation and attachment. To study how biofilms might form on actual components, we submerged multiple orthopedic implants of various shapes, sizes, roughness and material type in brain heart infusion broth inoculated with Staphylococcus aureus SAP231, a bioluminescent USA300 strain. Implants were incubated for 72 h with daily media exchanges. After incubation, implants were imaged using an in vitro imaging system (IVIS) and the metabolic signal produced by biofilms was quantified by image analysis. Scanning electron microscopy was then used to image different areas of the implants to complement the IVIS imaging. Rough surfaces had the greatest luminescence compared to edges or smooth surfaces on a single implant and across all implants when the images were merged. The luminescence of edges was also significantly greater than smooth surfaces. These data suggest implant roughness, as well as large-scale surface features, may be at greater risk of biofilm colonization.
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影响因子：
3.1
作者：
McConoughey SJ;Howlin R;Granger JF;Manring MM;Calhoun JH;Shirtliff M;Kathju S;Stoodley P
通讯作者：
Stoodley P
影响因子：
4
作者：
Jeong, Won-Seok;Kwon, Jae-Sung;Kim, Kwang-Mahn
通讯作者：
Kim, Kwang-Mahn
影响因子：
4.4
作者：
Gupta, Tripti Thapa;Gupta, Niraj K.;Stoodley, Paul
通讯作者：
Stoodley, Paul
影响因子：
3.7
作者：
Bernthal NM;Stavrakis AI;Billi F;Cho JS;Kremen TJ;Simon SI;Cheung AL;Finerman GA;Lieberman JR;Adams JS;Miller LS
通讯作者：
Miller LS
影响因子：
4.9
作者：
Kuehl, Richard;Brunetto, Priscilla S.;Khanna, Nina
通讯作者：
Khanna, Nina