Diversity of cultivable methane-oxidizing bacteria in microsites of a rice paddy field: investigation by cultivation method and fluorescence in situ hybridization (FISH).
Diversity of cultivable methane-oxidizing bacteria in microsites of a rice paddy field: investigation by cultivation method and fluorescence in situ hybridization (FISH).
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DOI：
10.1264/jsme2.me11327
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发表时间：
2012
影响因子：
2.2
通讯作者：
Asakawa S
中科院分区：
文献类型：
--
作者：
Dianou D;Ueno C;Ogiso T;Kimura M;Asakawa S
通过培养依赖性和荧光原位杂交（FISH）技术相结合，研究了稻田生态系统中可培养的甲烷氧化细菌（MOB）的多样性。日本稻田的七个微型地点是研究的重点：洪水、表层土壤、大块土壤、根际土壤、根部、水稻植株的基茎和上一收获的稻茬。基于 pmoA 基因分析和透射电子显微镜 (TEM)，从最高稀释系列的富集培养物中获得了 4 种 I 型和 9 种 II 型 MOB 分离株。 I 型 MOB 分离株包括洪水中甲基单胞菌属的一个新物种，这是第一个从稻田洪水中分离出来的 I 型 MOB 菌株。在 I 型 MOB 中，来自树桩的两个分离株与甲基单胞菌属密切相关；从根际土壤中获得的一种分离物与甲基球菌-甲基球菌-甲基球菌分支最相关。几乎所有 II 型 MOB 分离株都与甲基囊藻甲烷氧化菌有关。 FISH 证实所有微位点和相关富集培养物中均存在 I 型和 II 型 MOB。该研究首次报告了稻田隔间中可培养的甲烷氧化菌的多样性，包括 I 型 MOB 的新物种。改进生长介质和培养条件，结合分子方法，将使我们能够扩大对稻田生态系统中 MOB 群落的了解，从而实施减少该生态系统 CH4 排放的策略。
The diversity of cultivable methane-oxidizing bacteria (MOB) in the rice paddy field ecosystem was investigated by combined culture-dependent and fluorescence in situ hybridization (FISH) techniques. Seven microsites of a Japanese rice paddy field were the focus of the study: floodwater, surface soil, bulk soil, rhizosphere soil, root, basal stem of rice plant, and rice stumps of previous harvest. Based on pmoA gene analysis and transmission electron microscopy (TEM), four type I, and nine type II MOB isolates were obtained from the highest dilution series of enrichment cultures. The type I MOB isolates included a novel species in the genus Methylomonas from floodwater and this is the first type I MOB strain isolated from floodwater of a rice paddy field. In the type I MOB, two isolates from stumps were closely related to Methylomonas spp.; one isolate obtained from rhizosphere soil was most related to Methyloccocus-Methylocaldum-Methylogaea clade. Almost all the type II MOB isolates were related to Methylocystis methanotrophs. FISH confirmed the presence of both types I and II MOB in all the microsites and in the related enrichment cultures. The study reported, for the first time, the diversity of cultivable methanotrophs including a novel species of type I MOB in rice paddy field compartments. Refining growth media and culture conditions, in combination with molecular approaches, will allow us to broaden our knowledge on the MOB community in the rice paddy field ecosystem and consequently to implement strategies for mitigating CH4 emission from this ecosystem.
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影响因子：
4.4
作者：
BOWMAN, JP;JIMENEZ, L;SAYLER, GS
通讯作者：
SAYLER, GS
影响因子：
4.2
作者：
Bourne, DG;Holmes, AJ;Murrell, JC
通讯作者：
Murrell, JC
影响因子：
4.2
作者：
Bussmann, I;Pester, M;Schink, B
通讯作者：
Schink, B
影响因子：
4.4
作者：
Eller, G;Frenzel, P
通讯作者：
Frenzel, P
影响因子：
2.2
作者：
Amalfitano, Stefano;Fazi, Stefano
通讯作者：
Fazi, Stefano