Arp5 is a key regulator of myocardin in smooth muscle cells.
Arp5 is a key regulator of myocardin in smooth muscle cells.
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DOI：
10.1083/jcb.201307158
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发表时间：
2014-03-03
期刊：
影响因子：
--
通讯作者：
Hayashi K
中科院分区：
文献类型：
--
作者：
Morita T;Hayashi K
Arp5 suppresses myocardin activity through both direct binding to myocardin and binding to SRF to prevent transcriptional activation of myogenic genes by the myocardin–SRF complex. Myocardin (Myocd) and Myocd-related transcription factors (MRTFs) are robust coactivators of serum response factor (SRF). RPEL motifs are monomeric globular actin (G-actin) binding elements that regulate MRTF localization and activity. However, the function of the RPEL motif in Myocd is largely unknown because of its low affinity for G-actin. Here, we demonstrated that the Myocd RPEL motif bound to actin-related protein 5 (Arp5) instead of conventional actin, resulting in a significant suppression of Myocd activity. In addition, Arp5 bound to a DNA binding domain of SRF via its C-terminal sequence and prevented the association of the Myocd–SRF complex with the promoter regions of smooth muscle genes. Well-differentiated smooth muscle cells mainly expressed a specific splicing variant of arp5; therefore, the protein level of Arp5 was markedly reduced by partial messenger RNA decay and translational suppression. In dedifferentiated smooth muscle cells, Arp5 knockdown restored the differentiated phenotype via Myocd activation. Thus, Arp5 is a key regulator of Myocd activity.
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DOI：
10.1016/s1937-6448(09)77005-4
发表时间：
2009
影响因子：
--
作者：
Meagher, Richard B.;Kandasamy, Muthugapatti K.;McKinney, Elizabeth C.;Roy, Eileen
通讯作者：
Roy, Eileen
DOI：
10.1073/pnas.1233635100
发表时间：
2003-08-05
影响因子：
11.1
作者：
Li, SJ;Wang, DZ;Olson, EN
通讯作者：
Olson, EN
DOI：
10.1038/nrm3454
发表时间：
2012-11
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
11.8
作者：
Liu, ZP;Wang, ZG;Olson, EN
通讯作者：
Olson, EN
影响因子：
5.3
作者：
Oh, J;Wang, ZG;Olson, EN
通讯作者：
Olson, EN