TGFβ2 differentially modulates smooth muscle cell proliferation and migration in electrospun gelatin-fibrinogen constructs.
TGFβ2 differentially modulates smooth muscle cell proliferation and migration in electrospun gelatin-fibrinogen constructs.
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TGFβ2差异地调节了平滑肌细胞的增殖和电纺明胶 - 纤维蛋白原构建体中的迁移。
DOI：
10.1016/j.biomaterials.2014.10.021
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发表时间：
2015-01
期刊：
影响因子：
14
通讯作者：
Vande Geest, Jonathan P.
中科院分区：
文献类型：
--
作者：
Ardila, Diana C.;Tamimi, Ehab;Danford, Forest L.;Haskett, Darren G.;Kellar, Robert S.;Doetschman, Tom;Vande Geest, Jonathan P.
组织工程的一个主要目标是开发替代、恢复和改善受损组织的支架。这些支架必须模拟天然组织，由细胞外基质（ECM）支持物、附着于ECM的细胞和信号分子（如调节细胞功能的生长因子）构成。在这项研究中，我们使用不同的明胶和纤维蛋白原的组合物创建静电纺丝平板支架。将平滑肌细胞（SMCs）接种在支架上，并评价增殖和浸润。此外，向培养基中加入不同浓度的转化生长因子-β 2（TGFβ2），目的是阐明其对细胞增殖、迁移和胶原蛋白产生的影响。我们的研究结果表明，具有80%明胶-20%纤维蛋白原的组成的支架适合于组织工程应用，因为它促进细胞生长和迁移。低浓度（≤ 1 ng/ml）TGFβ2可促进SMC增殖和支架浸润，减少胶原生成。相反，浓度> 1 ng/ml的TGFβ2抑制细胞增殖和迁移，同时刺激胶原生成。根据我们的研究结果，TGFβ2浓度对SMC功能具有不同的影响，因此可以用作有利于组织工程应用的生化调节剂。
A main goal of tissue engineering is the development of scaffolds that replace, restore and improve injured tissue. These scaffolds have to mimic natural tissue, constituted by an extracellular matrix (ECM) support, cells attached to the ECM, and signaling molecules such as growth factors that regulate cell function. In this study we created electrospun flat sheet scaffolds using different compositions of gelatin and fibrinogen. Smooth muscle cells (SMCs) were seeded on the scaffolds, and proliferation and infiltration were evaluated. Additionally, different concentrations of Transforming Growth Factor-beta2 (TGFβ2) were added to the medium with the aim of elucidating its effect on cell proliferation, migration and collagen production. Our results demostrated that a scafold with a composition of 80% gelatin-20% fibrinogen is suitable for tissue engineering applications since it promotes cell growth and migration. The addition of TGFβ2 at low concentrations (≤1ng/ml) to the culture medium resulted in an increase in SMC proliferation and scaffold infiltration, and in the reduction of collagen production. In contrast, TGFβ2 at concentrations >1ng/ml inhibited cell proliferation and migration while stimulating collagen production. According to our results TGFβ2 concentration has a differential effect on SMC function and thus can be used as a biochemical modulator that can be beneficial for tissue engineering applications.
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影响因子：
2.5
作者：
Azhar, Mohamad;Brown, Kristen;Gard, Connie;Chen, Hwudaurw;Rajan, Sudarsan;Elliott, David A.;Stevens, Mark V.;Camenisch, Todd D.;Conway, Simon J.;Doetschman, Thomas
通讯作者：
Doetschman, Thomas
影响因子：
20.1
作者：
Gollasch, M;Ried, C;Haller, H
通讯作者：
Haller, H
DOI：
10.1016/j.msec.2006.05.019
发表时间：
2007-04-01
期刊：
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
影响因子：
--
作者：
Lannutti, J.;Reneker, D.;Farson, D. F.
通讯作者：
Farson, D. F.
影响因子：
2.2
作者：
Kubota, K;Okazaki, J;Liu, B
通讯作者：
Liu, B
影响因子：
5.3
作者：
ISHIDA, T;TANAKA, K
通讯作者：
TANAKA, K