Localization of relaxin in the pig follicle during preovulatory development.
Localization of relaxin in the pig follicle during preovulatory development.
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排卵前发育期间猪卵泡中松弛素的定位。
DOI：
10.1095/biolreprod37.1.235
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发表时间：
1987
影响因子：
3.6
通讯作者：
Ainsworth,L
中科院分区：
文献类型：
--
作者：
Bagnell,CA;Frando,LB;Downey,BR;Tsang,BK;Ainsworth,L
采用亲和素-生物素免疫过氧化物酶法和纯化猪松弛素抗血清，对妊娠母马血清促性腺激素(PMSG)/人绒毛膜促性腺激素（hCG）诱导的猪卵泡切片进行松弛素定位。用PMSG （750 IU）和hCG （500 IU）分别诱导青春期前猪的卵泡发育和排卵。在PMSG处理后24、48、60、72、84、96或108小时，从未处理的后备母猪或内脏中收集卵泡。在卵泡发育早期（pmsg后48和60 h）观察到卵泡内腔的光免疫染色。pmsg后72 h，排卵前卵泡内膜松弛素免疫染色更强烈。经hCG治疗后，强烈的鞘膜免疫染色持续存在，并在PMSG后84和96 h明显。在预期排卵前约6小时（pmsg后108小时），卵泡壁变薄，卵膜内松弛素免疫染色减少。在研究的任何时间点，未处理的后备母猪的卵泡、pmsg后24小时的卵泡、健康或闭锁的小卵泡、颗粒细胞、外膜或卵巢间质中均未检测到免疫活性松弛素。这些研究支持了卵泡内膜是卵泡松弛素主要来源的假设，并为松弛素在排卵过程中的旁分泌作用提供了进一步的证据。
The avidin-biotin immunoperoxidase method and antisera to purified porcine relaxin were used to localize relaxin in sections of follicles from pregnant mare’s serum gonadotropin(PMSG)/human chorionic gonadotropin (hCG)-primed pigs during preovulatory development. Prepubertal pigs were treated im with PMSG (750 IU) and 72 h later with hCG (500 IU) to induce follicular development and ovulation. Follicles were collected from untreated gilts or from guts 24, 48, 60, 72, 84, 96, or 108 h after PMSG treatment. Light immunostaining in the theca interna was observed early in follicular development, at 48 and 60 h post-PMSG. At 72 h post-PMSG, relaxin immunostaining in the theca interna of the preovulatory follicle was more intense. After hCG treatment, the intense thecal immunostaining persisted and was apparent 84 and 96 h after PMSG. At about 6 h prior to expected ovulation(108 h post-PMSG), there was thinning of the follicle wall and a reduction in relaxin immunostaining in the theca interna. Immunoactive relaxin was not detected in follicles from untreated gilts, follicles 24 h post-PMSG, small healthy or atretic follicles, or in granulosa cells, theca externa or ovarian stroma, at any of the time points studied. These studies support the hypothesis that the theca interna is the primary source of follicular relaxin and provide further evidence for a paracrine role for relaxin in the ovulatory process.
猪排卵前期间卵泡膜松弛素产生的变化。
DOI：
10.1530/jrf.0.0690677
发表时间：
1983
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
G. Evans;D. Wathes;G. J. King;D. Armstrong;D. G. Porter
通讯作者：
D. G. Porter
类风湿滑膜细胞对潜在胶原酶的内源性激活。
DOI：
10.1056/nejm197705052961801
发表时间：
1977
期刊：
The New England journal of medicine
影响因子：
--
作者：
Z. Werb;C. Mainardi;C. Vater;E. D. Harris
通讯作者：
E. D. Harris
猪正常排卵和诱导排卵期间卵子的成熟过程
影响因子：
3.3
作者：
J. F. Spalding;R. O. Berry;John Moffit
通讯作者：
John Moffit