Myocardial kinetics of 201Thallium, 99mTc-tetrofosmin, and 99mTc-sestamibi in an acute ischemia–reperfusion model using isolated rat heart
Myocardial kinetics of 201Thallium, 99mTc-tetrofosmin, and 99mTc-sestamibi in an acute ischemia–reperfusion model using isolated rat heart
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使用离体大鼠心脏的急性缺血再灌注模型中 201Thallium、99mTc-tetrofosmin 和 99mTc-sestamibi 的心肌动力学
DOI：
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发表时间：
2007
影响因子：
2.6
通讯作者：
H. Kasanuki
中科院分区：
文献类型：
--
作者：
K. Fukushima;M. Momose;C. Kondo;K. Kusakabe;H. Kasanuki
铊（TL）、99 mTc-tetrofosmin（TF）和99 mTc-sestamibi（MIBI）被广泛用作心肌灌注剂。本研究的目的是评估其动力学在急性缺血-再灌注。MethodsIsolated大鼠心脏，灌流的Langendorff方法在10 ml/min的恒定流速，被分配到正常对照组，轻度缺血，和严重缺血组，其中20分钟的示踪剂冲洗进行了25分钟的示踪剂冲洗。缺血组在进行洗入和洗出之前诱导无血流缺血（轻度缺血组15 min;重度缺血组30 min）。使用外部伽马检测器测定全心脏放射性。心肌流速（K1，ml/min）和清除率结果K1 TL、K1 TF、K1 MIBI随缺血程度的加重而降低（K1 TL 5.32 ± 0.53、4.76 ± 0.70和1.44 ± 0.59; K1 TF 3.80 ± 0.70、2.73 ± 0.99和1.09 ± 0.45;正常对照组、轻度缺血组和重度缺血组的K1 MIBI分别为3.45 ± 1.10、2.15 ± 0.82和1.05 ± 0.13）。TL的K1显著高于99 mTc示踪剂（P < 0.05），但99 mTc示踪剂的K1值相当。缺血组k2 TL显著增加（P < 0.05）（k2 TL 0.062 ± 0.013，0.11 ± 0.045，0.12 ± 0.035），但缺血组间无显著性差异。k2 MIBI和k2 TF在正常人中（P < 0.05）低于k2 TL，显著升高重度缺血组（P < 0.05）（k2 TF 0.0056 ± 0.0022、0.0037 ± 0.0015和0.024 ± 0.015;和k2 MIBI 0.00072 ± 0.0011、0.00038 ± 0.00076和0.042 ± 0.034）。在正常对照组和轻度缺血组中，k2 MIBI显著低于k2 TF（P <0.0 5）。结论TL的示踪剂提取率高于99 mTc示踪剂，三个示踪剂组的示踪剂提取率均随缺血再灌注的严重程度而降低。清除动力学的MIBI，但也TF可能是有用的缺血的严重程度的评价，Langendorff方法和方法学的方法，通过连续测定放射性可用于示踪剂动力学曲线的定量分析。
Objective201Thallium (TL), 99mTc-tetrofosmin (TF), and 99mTc-sestamibi (MIBI) are extensively used as myocardial perfusion agents. The objective of the present study was to evaluate their kinetics under acute ischemia–reperfusion.MethodsIsolated rat hearts, perfused by the Langendorff method at a constant flow rate of 10 ml/min, were allotted to normal control, mild ischemia, and severe ischemia groups, in which 20-min tracer wash-in was conducted followed by a 25-min tracer washout. No-flow ischemia (15 min for mild ischemia groups; 30 min for severe ischemia groups) was induced before conducting wash-in and washout in the ischemia groups. Whole-heart radioactivity was determined with an external gamma detector. Myocardial flow rate (K1, ml/min) and clearance rate (k2, min−1) were calculated.ResultsK1TL, K1TF, and K1MIBI decreased according to the severity of ischemia (K1TL 5.32 ± 0.53, 4.76 ± 0.70, and 1.44 ± 0.59; K1TF 3.80 ± 0.70, 2.73 ± 0.99, and 1.09 ± 0.45; and K1MIBI 3.45 ± 1.10, 2.15 ± 0.82, and 1.05 ± 0.13, in the normal control, mild, and severe ischemia groups, respectively). K1 was significantly higher for TL than for the 99mTc tracers (P < 0.05), but the 99mTc tracers had equivalent K1 values. k2TL increased significantly (P < 0.05) in the ischemia groups (k2TL 0.062 ± 0.013, 0.11 ± 0.045, and 0.12 ± 0.035), but showed no significant difference between the ischemia groups. k2MIBI and k2TF were significantly (P < 0.05) lower than k2TL and increased significantly (P < 0.05) in the severe ischemia group (k2TF 0.0056 ± 0.0022, 0.0037 ± 0.0015, and 0.024 ± 0.015; and k2MIBI 0.00072 ± 0.0011, 0.00038 ± 0.00076, and 0.042 ± 0.034). k2MIBI was significantly (P < 0.05) lower than k2TF in the normal control and mild ischemia groups.ConclusionsTracer extraction was higher for TL than for the 99mTc tracers and all tracers decreased according to the severity of ischemia–reperfusion in the three tracer groups. The clearance kinetics of not only MIBI but also TF is possibly useful for the evaluation of the severity of ischemia, and the Langendorff method and a methodological approach by continuous determinations of radioactivity may serve for the quantitative analysis of tracer kinetic profiles.
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影响因子：
20.1
作者：
Asimakis,GK;Sandhu,GS;Conti,VR;Sordahl,LA;Zwischenberger,JB
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影响因子：
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影响因子：
37.8
作者：
Moore,CA;Cannon,J;Watson,DD;Kaul,S;Beller,GA
通讯作者：
Beller,GA
影响因子：
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作者：
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通讯作者：
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DOI：
--
发表时间：
1993
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
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作者：
Dahlberg,ST;Meerdink,DJ;Gilmore,MP;Leppo,JA
通讯作者：
Leppo,JA