Coupled Chromatography for Assay of the Venom Proteome of the Snake Agkistrodon acutus: An Effective Strategy for Discovery of Active Components
Coupled Chromatography for Assay of the Venom Proteome of the Snake Agkistrodon acutus: An Effective Strategy for Discovery of Active Components
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耦合色谱法测定蛇毒蛋白质组：发现活性成分的有效策略
DOI：
10.1365/s10337-009-0987-1
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发表时间：
2009-02
期刊：
影响因子：
1.7
通讯作者：
Huang, Xiaodong
中科院分区：
文献类型：
--
作者：
Kong, Yi;Wu, Wutong;Huang, Chunhong;Huang, Xiaodong
二维液相色谱（2D-LC）是蛋白质组分析的一个有吸引力的选择，因为它在分离和分析极端pH和分子量的蛋白质和肽方面具有优势。蛋白质组学被认为是发现新的活性天然产物的一种有前途的高通量方法，它要求综合分离技术的进步，特别是对肽，糖蛋白，疏水蛋白等。在这里，一个优化的离线IEX-RP LC系统已被用于分离五步蛇（尖吻蝮蛇）毒液的所有组分。利用该系统共获得79种天然蛇毒组分，比双向电泳所获得的组分多。一个自动化的在线IEX-RP LC系统也被开发用于感兴趣的组分的心切分离。结果表明，在蛇毒中发现了一种新的三肽（PGlu-Asn-Trp，分子量429.1）。这强调了2D-LC在药物发现中的作用。
Two-dimensional liquid chromatography (2D-LC) is an attractive option for proteome profiling because of its advantages in separation and analysis of proteins and peptides of extreme pH and molecular weight. Proteomics, regarded as a promising and high-throughput method for discovery of new active natural products, calls for technical progress in comprehensive separation, especially for peptides, glycoproteins, and hydrophobic proteins, etc. Here, an optimized off-line IEX-RP LC system has been used for separation of all the components of the venom of the five-pace snake (Agkistrodon acutus). Seventy-nine different natural venom components were obtained by use of this system, more than were obtained by two dimensional electrophoresis of the venom. An automated on-line IEX-RP LC system was also developed for heart-cut separation of components of interest. As a result, we discovered a new tripeptide (PGlu-Asn-Trp, MW 429.1) in the venom. This emphasizes the role of 2D-LC in drug discovery.
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DOI：
10.1016/s1570-0232(02)00554-8
发表时间：
2002-12
期刊：
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
影响因子：
--
作者：
Hongji Liu;S. Berger;Asish B. Chakraborty;R. Plumb;S. Cohen
通讯作者：
Hongji Liu;S. Berger;Asish B. Chakraborty;R. Plumb;S. Cohen
DOI：
10.1016/j.bbrc.2006.01.006
发表时间：
2006-03-10
影响因子：
3.1
作者：
Liu, QH;Zhang, XW;Yan, GM
通讯作者：
Yan, GM
影响因子：
2.9
作者：
Candiano, G;Bruschi, M;Righetti, PG
通讯作者：
Righetti, PG
影响因子：
2.8
作者：
Oyama, Etsuko;Takahashi, Hidenobu
通讯作者：
Takahashi, Hidenobu
影响因子：
7
作者：
Jiang, XS;Zhou, H;Zeng, R
通讯作者：
Zeng, R