Hyperosmotic priming of Arabidopsis seedlings establishes a long-term somatic memory accompanied by specific changes of the epigenome.
Hyperosmotic priming of Arabidopsis seedlings establishes a long-term somatic memory accompanied by specific changes of the epigenome.
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DOI：
10.1186/gb-2013-14-6-r59
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发表时间：
2013-06-14
期刊：
影响因子：
12.3
通讯作者：
Amtmann A
中科院分区：
文献类型：
--
作者：
Sani E;Herzyk P;Perrella G;Colot V;Amtmann A
在干旱和半干旱环境中，干旱和土壤盐碱化通常发生在植物生命周期的开始和结束时，这为引发幼苗以增强成熟植物的胁迫耐受性提供了自然机会。染色质标记，如组蛋白修饰，提供了一个潜在的分子机制，引发植物的环境压力，但是否短暂的高渗胁迫暴露的幼苗导致染色质的变化，维持整个营养生长仍不清楚。我们已经建立了一个有效的协议，高渗引发的模式植物拟南芥，其中包括一个短暂的温和的盐处理的幼苗，然后在控制条件下的广泛的一段时间的增长。引发的植物在生长和发育方面与未引发的植物相同，但它们在第二次胁迫暴露后显示出盐吸收减少和耐旱性增强。四种组蛋白修饰的ChIP-seq分析显示，引发处理改变了表观基因组景观;变化很小，但它们对处理的组织具有特异性，根据修饰的数量和方向而变化，并且优先靶向转录因子。值得注意的是，引发导致H3 K27 me 3岛的缩短和分馏。这种效应会随着时间的推移而减弱，但在对照条件下经过十天的生长期后仍然明显。在H3 K27 me 3中具有引发诱导的差异的几个基因显示对第二应激处理的转录响应性改变。幼植物对瞬时高渗胁迫的经历被储存在一个长期的体细胞记忆中，包括染色质状态、转录响应和整个植物生理的差异。
In arid and semi-arid environments, drought and soil salinity usually occur at the beginning and end of a plant's life cycle, offering a natural opportunity for the priming of young plants to enhance stress tolerance in mature plants. Chromatin marks, such as histone modifications, provide a potential molecular mechanism for priming plants to environmental stresses, but whether transient exposure of seedlings to hyperosmotic stress leads to chromatin changes that are maintained throughout vegetative growth remains unclear. We have established an effective protocol for hyperosmotic priming in the model plant Arabidopsis, which includes a transient mild salt treatment of seedlings followed by an extensive period of growth in control conditions. Primed plants are identical to non-primed plants in growth and development, yet they display reduced salt uptake and enhanced drought tolerance after a second stress exposure. ChIP-seq analysis of four histone modifications revealed that the priming treatment altered the epigenomic landscape; the changes were small but they were specific for the treated tissue, varied in number and direction depending on the modification, and preferentially targeted transcription factors. Notably, priming leads to shortening and fractionation of H3K27me3 islands. This effect fades over time, but is still apparent after a ten day growth period in control conditions. Several genes with priming-induced differences in H3K27me3 showed altered transcriptional responsiveness to the second stress treatment. Experience of transient hyperosmotic stress by young plants is stored in a long-term somatic memory comprising differences of chromatin status, transcriptional responsiveness and whole plant physiology.
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影响因子：
7.2
作者：
Maathuis, FJM;Filatov, V;Amtmann, A
通讯作者：
Amtmann, A
DOI：
10.1073/pnas.1209329109
发表时间：
2012-08-07
影响因子：
11.1
作者：
Dowen, Robert H.;Pelizzola, Mattia;Ecker, Joseph R.
通讯作者：
Ecker, Joseph R.
DOI：
10.1073/pnas.0908755106
发表时间：
2009-11-24
影响因子：
11.1
作者：
Hauben, Miriam;Haesendonckx, Boris;De Block, Marc
通讯作者：
De Block, Marc
影响因子：
64.8
作者：
Ito, Hidetaka;Gaubert, Herve;Paszkowski, Jerzy
通讯作者：
Paszkowski, Jerzy
影响因子：
4.9
作者：
Kim, Jong-Myong;To, Taiko Kim;Seki, Motoaki
通讯作者：
Seki, Motoaki