Substantial differences in soil viral community composition within and among four Northern California habitats.
Substantial differences in soil viral community composition within and among four Northern California habitats.
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DOI：
10.1038/s43705-022-00171-y
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发表时间：
2022-10-13
期刊：
影响因子：
--
通讯作者：
Emerson, Joanne B.
中科院分区：
文献类型：
--
作者：
Durham, Devyn M.;Sieradzki, Ella T.;ter Horst, Anneliek M.;Santos-Medellin, Christian;Bess, C. Winston A.;Geonczy, Sara E.;Emerson, Joanne B.
病毒有助于不同生态系统中的食物网动态和养分循环，但人们对这些病毒动态背后的生物地理模式知之甚少，特别是在土壤中。在这里，我们确定了土壤病毒群落组成与栖息地、水分含量和物理距离相关的趋势。我们通过在提取 DNA 之前选择性捕获病毒大小的颗粒，从四个不同的栖息地（湿地、草原、林地和灌木丛）生成了 30 个土壤病毒组，并回收了 3432 个独特的病毒“物种”（dsDNA vOTU）。病毒群落因土壤湿度而存在显着差异，与干燥土壤栖息地相比，潮湿土壤栖息地的病毒丰富度通常更高。然而，vOTU 很少在病毒体之间共享，包括重复间隔<10米，这表明土壤病毒可能无法很好地分散，未来的土壤病毒群落采样策略可能需要考虑小空间尺度上的极端群落差异。在超过一种病毒组中检测到的 19% vOTU 中，93% 来自相同的栖息地和地点，这表明在更接近的环境条件下，病毒群落的相似性更大。栖息地内的差异表明，需要进行广泛的采样才能进行严格的跨栖息地比较，结果强调了潮湿土壤中高病毒活性和土壤病毒群落空间异质性的新兴范例。
Viruses contribute to food web dynamics and nutrient cycles in diverse ecosystems, yet the biogeographical patterns that underlie these viral dynamics are poorly understood, particularly in soil. Here, we identified trends in soil viral community composition in relation to habitat, moisture content, and physical distance. We generated 30 soil viromes from four distinct habitats (wetlands, grasslands, woodlands, and chaparral) by selectively capturing virus-sized particles prior to DNA extraction, and we recovered 3432 unique viral ‘species’ (dsDNA vOTUs). Viral communities differed significantly by soil moisture content, with viral richness generally higher in wet compared to dry soil habitats. However, vOTUs were rarely shared between viromes, including replicates <10 m apart, suggesting that soil viruses may not disperse well and that future soil viral community sampling strategies may need to account for extreme community differences over small spatial scales. Of the 19% of vOTUs detected in more than one virome, 93% were from the same habitat and site, suggesting greater viral community similarity in closer proximity and under similar environmental conditions. Within-habitat differences indicate that extensive sampling would be required for rigorous cross-habitat comparisons, and results highlight emerging paradigms of high viral activity in wet soils and soil viral community spatial heterogeneity.
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影响因子：
15.5
作者：
Ter Horst AM;Santos-Medellín C;Sorensen JW;Zinke LA;Wilson RM;Johnston ER;Trubl G;Pett-Ridge J;Blazewicz SJ;Hanson PJ;Chanton JP;Schadt CW;Kostka JE;Emerson JB
通讯作者：
Emerson JB
影响因子：
9.7
作者：
Kuzyakov, Yakov;Mason-Jones, Kyle
通讯作者：
Mason-Jones, Kyle
DOI：
10.1073/pnas.2105124118
发表时间：
2021-08-10
影响因子：
11.1
作者：
Lee, Sungeun;Sieradzki, Ella T.;Nicol, Graeme W.
通讯作者：
Nicol, Graeme W.
影响因子：
5.2
作者：
Roy, Krishnakali;Ghosh, Dhritiman;Radosevich, Mark
通讯作者：
Radosevich, Mark
DOI：
10.1038/s43705-022-00110-x
发表时间：
2022
期刊：
ISME communications
影响因子：
--
作者：
Hillary LS;Adriaenssens EM;Jones DL;McDonald JE
通讯作者：
McDonald JE