Intact cag pathogenicity island of Helicobacter pylori without disease association in Kolkata, India.
Intact cag pathogenicity island of Helicobacter pylori without disease association in Kolkata, India.
复制标题
DOI：
10.1016/j.ijmm.2010.10.003
复制
发表时间：
2011-04
影响因子：
4.1
通讯作者：
Mukhopadhyay, Asish K.
中科院分区：
文献类型：
--
作者：
Patra, Rajashree;Chattopadhyay, Santanu;De, Ronita;Datta, Simanti;Chowdhury, Abhijit;Ramamurthy, T.;Nair, G. Balakrish;Berg, Douglas E.;Mukhopadhyay, Asish K.
幽门螺杆菌 cag 致病性岛 (cag PAI) 中的 cagA 等多个基因被认为与胃十二指肠疾病相关，因此 cag PAI 遗传结构的变异可能导致不同的临床结果。我们的研究旨在表征来自印度加尔各答十二指肠溃疡 (DU) 患者和无症状或非溃疡性消化不良 (NUD/AV) 受试者的幽门螺杆菌菌株的 cag PAI。使用 83 个不同引物对整个 cag PAI 进行 PCR 分析，并通过斑点印迹杂交分析从 52 个个体（30 个 DU 和 22 个 NUD/AV）分离的菌株。与从印度其他地区分离的幽门螺杆菌菌株不同，本研究中使用的菌株中有82.6%具有完整的cag PAI，9.6%的菌株具有部分删除的cag PAI，7.7%的菌株缺乏整个cag PAI。点印迹杂交在 HP0522-523 和 HP0532-HP0534 基因的 PCR 阴性菌株中分别产生了 100% 和 93.8% 的阳性信号。在所有 cagA 阳性菌株中也检测到完整的 cagA 启动子区域。此外，通过 RT-PCR 证实了来自 DU 和 NUD/AV 受试者的代表性菌株的 cagA mRNA 表达，表明这些菌株的活性 cagA 启动子区域。总共 66.7% 的加尔各答菌株产生的 HP0527 基因（virB10 的同源物）的扩增子比标准菌株 26695 短约 390 bp。然而，序列分析证实，删除并没有改变该基因的阅读框，并且通过RT-PCR分析检测到了mRNA转录本。蛋白质印迹分析显示，从 DU 和 NUD/AV 中分离的菌株表达 CagA 蛋白，并具有功能性 IV 型分泌系统。有趣的是，DU 和 NUD/AV 个体之间的 cag PAI 遗传结构没有发现显着差异，这表明其他细菌毒力因素、宿主易感性和环境决定因素至少在某些地理位置也会影响疾病结果。
Several genes including the cagA in the cag pathogenicity island (cag PAI) of Helicobacter pylori are thought to be associated with the gastroduodenal diseases and hence variation in the genetic structure of the cag PAI might be responsible for different clinical outcomes. Our study was undertaken to characterize the cag PAI of H. pylori strains from duodenal ulcer (DU) patients and asymptomatic or non-ulcer dyspepsia (NUD/AV) subjects from Kolkata, India. Strains isolated from 52 individuals (30 DU and 22 NUD/AV) were analyzed by PCR using 83 different primers for the entire cag PAI and also by dot-blot hybridization. Unlike H. pylori strains isolated from other parts of India, 82.6% of the strains used in this study had intact cag PAI, 9.6% had partially deleted cag PAI, and 7.7% of the strains lacked the entire cag PAI. Dot-blot hybridization yielded positive signals in 100% and 93.8% of PCR-negative strains for HP0522-523 and HP0532-HP0534 genes, respectively. An intact cagA promoter region was also detected in all cagA-positive strains. Furthermore, the expression of cagA mRNA was confirmed by RT-PCR for the representative strains from both DU and NUD/AV subjects indicating the active cagA promoter regions of these strains. A total of 66.7% of Kolkata strains produced a ~390-bp shorter amplicon than the standard strain 26695 for the HP0527 gene, homologue of virB10. However, sequence analyses confirmed that the deletion did not alter the reading frame of the gene, and mRNA transcripts were detected by RT-PCR analysis. The strains isolated from DU and NUD/AV express CagA protein and possess a functional type IV secretion system, as revealed by Western blot analyses. Interestingly, no significant differences in cag PAI genetic structure were found between DU and NUD/AV individuals suggesting that other bacterial virulence factors, host susceptibility, and environmental determinants also influence the disease outcome at least in certain geographical locations.
登录
查看更多内容
影响因子：
4.3
作者：
Ali, Mahaboob;Khan, Aleem A.;Habibullah, C. M.
通讯作者：
Habibullah, C. M.
DOI：
10.1073/pnas.90.12.5791
发表时间：
1993-06-15
影响因子：
11.1
作者：
COVACCI, A;CENSINI, S;RAPPUOLI, R
通讯作者：
RAPPUOLI, R
DOI：
10.1073/pnas.93.25.14648
发表时间：
1996-12-10
影响因子：
11.1
作者：
Censini, S;Lange, C;Covacci, A
通讯作者：
Covacci, A
影响因子：
9.4
作者：
Kauser, F;Khan, AA;Ahmed, N
通讯作者：
Ahmed, N
影响因子：
29.4
作者：
Atherton, JC;Peek, RM;Blaser, MJ
通讯作者：
Blaser, MJ