Automated 3D-Printed Microfluidic Array for Rapid Nanomaterial-Enhanced Detection of Multiple Proteins.
Automated 3D-Printed Microfluidic Array for Rapid Nanomaterial-Enhanced Detection of Multiple Proteins.
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DOI：
10.1021/acs.analchem.8b01198
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发表时间：
2018-06-19
影响因子：
7.4
通讯作者：
Rusling JF
中科院分区：
文献类型：
--
作者：
Kadimisetty K;Malla S;Bhalerao KS;Mosa IM;Bhakta S;Lee NH;Rusling JF
我们在这里报告了一种新型3D打印自动免疫阵列的制造和验证，以检测具有超低检测下限的多个蛋白质。这种低成本的微型免疫阵列采用了由CCD摄像机测量的电化学发光(ECL)检测，并采用微泵的触摸屏控制，以便于自动化使用。这种小型化的阵列具有预填充的储罐，将样品和试剂输送到纸张般薄的热解石墨微孔检测芯片，以完成夹心免疫分析。该检测芯片通过在微孔中使用单壁碳纳米管-抗体结合物以及使用大量标记的抗体修饰的RuBPY-二氧化硅纳米颗粒来产生ECL，从而实现了高灵敏度。一个阵列的总成本为0.65美元，一个八种蛋白质的分析可以一次两次进行，每种蛋白质的检测限为0.14美元，检测限低至稀释血清中78-110fg毫升的−1。电子控制系统的零部件成本为210美元。通过在25分钟内检测人血清样本中的八种蛋白前列腺癌生物标志物小组，证明了自动免疫阵列的实用性。该系统非常适合未来的临床和医疗保健点诊断测试，并可用于资源有限的环境。
We report here the fabrication and validation of a novel 3D-printed, automated immunoarray to detect multiple proteins with ultralow detection limits. This low cost, miniature immunoarray employs electrochemiluminescent (ECL) detection measured with a CCD camera and employs touch-screen control of a micropump to facilitate automated use. The miniaturized array features prefilled reservoirs to deliver sample and reagents to a paper-thin pyrolytic graphite microwell detection chip to complete sandwich immunoassays. The detection chip achieves high sensitivity by using single-wall carbon nanotube—antibody conjugates in the microwells and employing massively labeled antibody-decorated RuBPY—silica nanoparticles to generate ECL. The total cost of an array is $0.65, and an eight-protein assay can be done in duplicate for $0.14 per protein with limits of detection (LOD) as low as 78–110 fg mL−1 in diluted serum. The electronic control system costs $210 in components. Utility of the automated immunoarray was demonstrated by detecting an eight-protein prostate cancer biomarker panel in human serum samples in 25 min. The system is well suited to future clinical and point-of-care diagnostic testing and could be used in resource-limited environments.
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影响因子：
1.4
作者：
Chan, JM;Stampfer, MJ;Pollak, M
通讯作者：
Pollak, M
影响因子：
12.6
作者：
Chikkaveeraiah, Bhaskara V.;Mani, Vigneshwaran;Patel, Vyomesh;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
3D打印的流体设备中的裸露和DNA涂层的石墨电极处的电化学发光。
影响因子：
8.9
作者：
Bishop GW;Satterwhite-Warden JE;Bist I;Chen E;Rusling JF
通讯作者：
Rusling JF
影响因子：
8.9
作者：
Kadimisetty K;Malla S;Rusling JF
通讯作者：
Rusling JF
影响因子：
7.4
作者：
Gross, Bethany C.;Erkal, Jayda L.;Spence, Dana M.
通讯作者：
Spence, Dana M.