Two regulatory networks mediated by light and glucose involved in glycolytic gene expression in cyanobacteria.
Two regulatory networks mediated by light and glucose involved in glycolytic gene expression in cyanobacteria.
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由光和葡萄糖介导的两个调控网络参与蓝藻糖酵解基因的表达。
DOI：
10.1093/pcp/pcs115
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
M..
中科院分区：
文献类型：
--
作者：
Tabei;Y.;Okada;K.;Horii;E.;Mitsui;M.;Nagashima;Y.;Sakai;T.;Yoshida;T.;Kamiya;A.;Fujiwara;S.;Tsuzuki;M..
果糖1，6-二磷酸醛缩酶（FBA）是光合生物中参与糖酵解和光合作用的酶。在原核生物中，双向反应在相同的细胞区室（即细胞质）中进行。FBA基因fbaA的表达通过两个独立的途径诱导，由连续光和葡萄糖加脉冲光（GPL）刺激，在蓝细菌集胞藻（Synechocystissp.）PCC 6803。在GPL条件下，葡萄糖可以被甚至不在细胞中代谢的葡萄糖类似物替代。缺失突变体的转录本分析表明，组氨酸激酶，Hik 8，和响应调节，Sll 1330，发挥重要作用的信号成分infbaAexpression在GPL条件下，但不是在光合条件下。对sll 1330表达增强的细胞的分析表明，即使没有葡萄糖，fbaA表达也至少部分地被诱导，但为了进一步诱导，需要脉冲光刺激。这些结果证实了该藻的醛缩酶基因表达存在两条光依赖性调控途径。
Fructose 1,6-bisphosphate aldolase (FBA) is an enzyme involved in both glycolytic and photosynthetic reactions in photosynthetic organisms. In prokaryotes, the bidirectional reaction proceeds in the same cellular compartment, i.e. the cytoplasm. Expression of the FBA gene,fbaA, is induced through two independent pathways, stimulated by continuous light and by glucose plus pulsed light (GPL), in a cyanobactrium,Synechocystissp. PCC 6803. Under GPL conditions, glucose can be replaced by glucose analogs that are not even metabolized in a cell. Analyses of transcripts in deletion mutants suggested that both a histidine kinase, Hik8, and a response regulator, Sll1330, played important roles as signal components infbaAexpression under GPL conditions, but not under photosynthetic conditions. Analysis of a transformant in whichsll1330expression was enhanced demonstrated thatfbaAexpression was induced at least partially even without glucose, but for its further induction a pulsed light stimulus was required. These results substantiated that there are two light-dependent regulatory pathways for aldolase gene expression in this cyanobacterium.
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