Advancing full length genome sequencing for human RNA viral pathogens.

Advancing full length genome sequencing for human RNA viral pathogens.
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DOI:
10.2217/17460794.4.1.47
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发表时间:
2009-01
期刊:
影响因子:
3.1
通讯作者:
Spiro D
Spiro D
中科院分区:
医学4区
文献类型:
--
作者:
Djikeng A;Spiro D

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面对众多新出现和重新出现的病毒威胁,大规模基因组测序工作正在进行中,以实时监测病毒进化。为了充分了解病毒适应和进化的机制,并开发试剂和资源以更好地对新出现和重新出现的病毒感染进行分子诊断,人们越来越努力地生产全长病毒基因组序列。迄今为止,已经使用传统的基于桑格的测序开发了高通量平台,并且目前有望应用下一代测序方法来开发用于病毒基因组测序和分析的超高通量策略。
In the face of numerous emerging and re-emerging viral threats, large-scale genome sequencing efforts are underway to monitor viral evolution in real-time. To fully appreciate the mechanisms of viral adaptation and evolution, and to also develop reagents and resources for a better molecular diagnosis of emerging and re-emerging viral infections, there has been an increasing effort toward producing full length viral genome sequences. To date, high-throughput platforms have been developed using traditional Sanger-based sequencing and there are currently prospects to apply next generation sequencing methods to develop an ultra high-throughput strategy for viral genome sequencing and analysis.
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