The effects of irreversible electroporation (IRE) on nerves.
The effects of irreversible electroporation (IRE) on nerves.
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不可逆电穿孔 (IRE) 对神经的影响。
DOI：
10.1371/journal.pone.0018831
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发表时间：
2011-04-14
期刊：
影响因子：
3.7
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Li W;Fan Q;Ji Z;Qiu X;Li Z
如果一个关键的神经周围涉及肿瘤，根治性手术旨在治愈癌症必须牺牲神经。失去关键神经可能导致严重后果。尽管神经重建技术取得了令人印象深刻的进步，但神经功能的完全恢复和正常化仍然难以实现。虽然不可逆电穿孔（IRE）可能是一个有前途的选择，以治疗肿瘤附近或涉及的关键神经，IRE治疗后的神经的病理生理尚未明确界定。我们将IRE直接应用于大鼠坐骨神经以研究IRE对神经的长期影响。将10个3800 V/cm的方脉冲序列（每个100 µs长）直接施加到大鼠坐骨神经。在第I组（IRE）的每只动物中，应用该程序以产生约10 mm的治疗长度。在第II组（对照组）的每只动物中，电极仅直接应用于坐骨神经相同的时间。术后即刻、术后3天、1周、3、5、7和10周进行电生理、组织学和功能研究。电生理、组织学和功能学结果表明，IRE处理的神经在7周后可完全恢复。这一发现表明，相对于应用IRE脉冲完全消融肿瘤，涉及恶性肿瘤的神经保留。总之，IRE可能是一种有前途的治疗工具，用于任何涉及神经的肿瘤。
If a critical nerve is circumferentially involved with tumor, radical surgery intended to cure the cancer must sacrifice the nerve. Loss of critical nerves may lead to serious consequences. In spite of the impressive technical advancements in nerve reconstruction, complete recovery and normalization of nerve function is difficult to achieve. Though irreversible electroporation (IRE) might be a promising choice to treat tumors near or involved critical nerve, the pathophysiology of the nerve after IRE treatment has not be clearly defined. We applied IRE directly to a rat sciatic nerve to study the long term effects of IRE on the nerve. A sequence of 10 square pulses of 3800 V/cm, each 100 µs long was applied directly to rat sciatic nerves. In each animal of group I (IRE) the procedure was applied to produce a treated length of about 10 mm. In each animal of group II (Control) the electrodes were only applied directly on the sciatic nerve for the same time. Electrophysiological, histological, and functional studies were performed on immediately after and 3 days, 1 week, 3, 5, 7 and 10 weeks following surgery. Electrophysiological, histological, and functional results show the nerve treated with IRE can attain full recovery after 7 weeks. This finding is indicative of the preservation of nerve involving malignant tumors with respect to the application of IRE pulses to ablate tumors completely. In summary, IRE may be a promising treatment tool for any tumor involving nerves.
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影响因子：
11.2
作者：
Guo Y;Zhang Y;Klein R;Nijm GM;Sahakian AV;Omary RA;Yang GY;Larson AC
通讯作者：
Larson AC
DOI：
10.1016/j.bbrc.2008.08.060
发表时间：
2008-11-14
影响因子：
3.1
作者：
Tekle, E.;Wolfe, M. D.;Oubrahim, H.;Chock, P. B.
通讯作者：
Chock, P. B.
影响因子：
3.6
作者：
Hess, Jason R.;Brenner, Michael J.;Mackinnon, Susan E.
通讯作者：
Mackinnon, Susan E.
影响因子：
2.1
作者：
George, LT;Myckatyn, TM;Mackinnon, SE
通讯作者：
Mackinnon, SE
影响因子：
3.6
作者：
HARE, GMT;EVANS, PJ;HUNTER, DA
通讯作者：
HUNTER, DA