Generation of a 3D Liver Model Comprising Human Extracellular Matrix in an Alginate/Gelatin-Based Bioink by Extrusion Bioprinting for Infection and Transduction Studies.
Generation of a 3D Liver Model Comprising Human Extracellular Matrix in an Alginate/Gelatin-Based Bioink by Extrusion Bioprinting for Infection and Transduction Studies.
复制标题
DOI：
10.3390/ijms19103129
复制
发表时间：
2018-10-12
影响因子：
5.6
通讯作者：
Kurreck J
中科院分区：
文献类型：
--
作者：
Hiller T;Berg J;Elomaa L;Röhrs V;Ullah I;Schaar K;Dietrich AC;Al-Zeer MA;Kurtz A;Hocke AC;Hippenstiel S;Fechner H;Weinhart M;Kurreck J
生物打印是一种新技术，可以帮助克服与二维（2D）细胞培养和动物实验相关的限制，因为它允许生产由人类细胞组成的三维（3D）组织模型。本研究描述了由藻酸盐、明胶和人细胞外基质（hECM）组成的生物墨水的优化，以用气动挤出打印机打印人HepaRG肝细胞。测试所得组织模型用于研究腺相关病毒（AAV）载体转导和人腺病毒5（hAdV 5）感染的适用性。我们发现用0.5和lmg/mL hECM补充基本藻酸盐/明胶生物墨水为打印过程、打印的构建体的稳定性以及打印的HepaRG细胞的活力和代谢功能提供了期望的性质。通过血清型6的AAV载体有效地转导组织模型，其通过RNA干扰成功地沉默内源性靶标（亲环蛋白B）。此外，打印的3D模型支持有效的腺病毒复制，使其适合研究病毒生物学和开发新的抗病毒化合物。我们认为这里描述的方法是开发用于包括病毒载体和感染性病毒的研究的3D组织模型的范例。
Bioprinting is a novel technology that may help to overcome limitations associated with two-dimensional (2D) cell cultures and animal experiments, as it allows the production of three-dimensional (3D) tissue models composed of human cells. The present study describes the optimization of a bioink composed of alginate, gelatin and human extracellular matrix (hECM) to print human HepaRG liver cells with a pneumatic extrusion printer. The resulting tissue model was tested for its suitability for the study of transduction by an adeno-associated virus (AAV) vector and infection with human adenovirus 5 (hAdV5). We found supplementation of the basic alginate/gelatin bioink with 0.5 and 1 mg/mL hECM provides desirable properties for the printing process, the stability of the printed constructs, and the viability and metabolic functions of the printed HepaRG cells. The tissue models were efficiently transduced by AAV vectors of serotype 6, which successfully silenced an endogenous target (cyclophilin B) by means of RNA interference. Furthermore, the printed 3D model supported efficient adenoviral replication making it suitable to study virus biology and develop new antiviral compounds. We consider the approach described here paradigmatic for the development of 3D tissue models for studies including viral vectors and infectious viruses.
登录
查看更多内容
影响因子：
--
作者：
Basheer L;Kerem Z
通讯作者：
Kerem Z
影响因子：
4.8
作者：
Baldwin, A;Kingman, H;Marks, DI
通讯作者：
Marks, DI
影响因子：
3.8
作者：
Gunness, Patrina;Mueller, Daniel;Noor, Fozia
通讯作者：
Noor, Fozia
DOI：
10.3390/v20801530
发表时间：
2010
期刊：
Viruses
影响因子：
--
作者：
Bouvier NM;Lowen AC
通讯作者：
Lowen AC
影响因子：
4.2
作者：
Enger, PO;Thorsen, F;Hoover, F
通讯作者：
Hoover, F