Isolation, characterization, and transplantation of adult liver progenitor cells.
Isolation, characterization, and transplantation of adult liver progenitor cells.
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成人肝祖细胞的分离、表征和移植。
DOI：
10.1007/978-1-62703-317-6_4
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Oertel,Michael
中科院分区：
文献类型：
--
作者：
Yovchev,MladenI;Dabeva,MarianaD;Oertel,Michael
许多慢性肝病危及生命。 When the liver loses the ability to repair itself the only treatment currently available is liver transplant. However, there are not enough donors to treat all the patients.这就需要寻找利用干细胞和祖细胞的替代疗法来治疗这些患者并恢复其正常肝功能。肝祖细胞可以从不同发育阶段的肝脏（包括成年肝脏）中分离出来。在成年大鼠肝脏中，有明确的证据表明祖细胞（也称为“卵圆细胞”）源自鲱鱼管中的前体细胞，能够分化为肝细胞和胆管细胞。在实验模型中，可以在体外分离和增殖肝祖细胞，并用于修复患病的肝脏。利用祖细胞的第一步是在肝脏中鉴定它们，分离纯化的祖细胞部分，随后将其移植到患病肝脏中，以评估移植细胞的肝脏再增殖，并评估其临床应用潜力。本方案描述了从wt DPPIV + F344大鼠中分离非实质细胞（NPC），然后纯化“卵圆细胞”，免疫组织化学染色技术来表征这些细胞，将其移植到 retrorsine-treated mutant DPPIV−rats, as well as the enzyme histochemical staining for DPPIV to detect transplanted cells in the host liver.
Many chronic liver diseases are life-threatening. When the liver loses the ability to repair itself the only treatment currently available is liver transplant. However, there are not enough donors to treat all the patients. This requires the search of alternative therapies utilizing stem and progenitor cells for treatment of these patients and restoration of their normal liver function.Hepatic progenitor cells can be isolated from livers at different developmental stages including adult liver. In the adult rat liver, there is clear evidence that progenitor cells (also called “oval cells”) derive from precursors in the canals of Herring that are capable to differentiate into hepatocytes and bile duct cells. In experimental models, hepatic progenitor cells can be isolated and propagated in vitro and used for restoration of the diseased liver. The first step in utilization of progenitor cells is their identification in the liver, isolation of purified progenitor cell fractions, which are subsequently transplanted in the diseased liver for evaluation of liver repopulation by transplanted cells, and evaluation their potentials for clinical application.The present protocol describes the isolation of non-parenchymal cells (NPCs) from wt DPPIV+F344 rats, followed by purification of “oval cells”, immunohistochemical staining techniques to characterize these cells, their transplantation into retrorsine-treated mutant DPPIV−rats, as well as the enzyme histochemical staining for DPPIV to detect transplanted cells in the host liver.
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DOI：
--
发表时间：
1981
期刊：
Journal of the National Cancer Institute
影响因子：
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作者：
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1.3
作者：
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D. Shafritz
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DOI：
--
发表时间：
2007
期刊：
Wound Repair Regen. 15
影响因子：
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作者：
Yamamoto H;Togo S;et. al.
通讯作者：
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DOI：
10.1002/path.1700760213
发表时间：
1958-01-01
期刊：
JOURNAL OF PATHOLOGY AND BACTERIOLOGY
影响因子：
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作者：
WILSON, JW;LEDUC, EH
通讯作者：
LEDUC, EH
影响因子：
29.4
作者：
Oertel, Michael;Menthena, Anuradha;Shafritz, David A.
通讯作者：
Shafritz, David A.