Proteome analysis identifies the Dpr protein of Streptococcus mutans as an important factor in the presence of early streptococcal colonizers of tooth surfaces.
Proteome analysis identifies the Dpr protein of Streptococcus mutans as an important factor in the presence of early streptococcal colonizers of tooth surfaces.
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DOI：
10.1371/journal.pone.0121176
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Ansai T
中科院分区：
文献类型：
--
作者：
Yoshida A;Niki M;Yamamoto Y;Yasunaga A;Ansai T
口腔链球菌是牙齿表面的主要定植菌，变形链球菌是人类龋病的主要致病菌。许多蛋白质参与了变形链球菌单种生物膜的形成。本研究利用双向凝胶电泳法(2-DE)分析了牙齿表面的先驱定殖物戈登氏链球菌存在或不存在时所形成的变形链球菌生物膜的蛋白质表达谱。通过质谱分析鉴定变形链球菌蛋白后，分析其在戈登链霉菌存在下的表达情况。将变形链球菌接种戈登链霉菌DL1或不接种戈登链霉菌。用0.2-μL无孔膜对这两个种进行了分类。收集聚苯乙烯平板上的生物膜，用双向凝胶电泳法分离可溶性蛋白质。当变形链球菌在戈登链霉菌存在下形成生物膜时，前者的过氧化氢抗性蛋白DPR的表达比在没有先驱定殖者的情况下形成的生物膜高4.3倍。此外，我们使用变形链球菌抗氧化蛋白突变体与戈登链球菌和其他初始定殖者一起进行了竞争试验。戈登氏链霉菌和血链球菌对DPR基因敲除突变链球菌的生长均有明显的抑制作用。此外，细胞活力测试显示，与野生型菌株相比，与戈登链球菌共培养时，DPR缺陷突变体的活力显著减弱。因此，这些结果表明，DPR可能是变形链球菌在口腔早期定植的情况下在牙齿上存活所必需的蛋白质之一。
Oral streptococci are primary colonizers of tooth surfaces and Streptococcus mutans is the principal causative agent of dental caries in humans. A number of proteins are involved in the formation of monospecies biofilms by S. mutans. This study analyzed the protein expression profiles of S. mutans biofilms formed in the presence or absence of S. gordonii, a pioneer colonizer of the tooth surface, by two-dimensional gel electrophoresis (2-DE). After identifying S. mutans proteins by Mass spectrometric analysis, their expression in the presence of S. gordonii was analyzed. S. mutans was inoculated with or without S. gordonii DL1. The two species were compartmentalized using 0.2-μl Anopore membranes. The biofilms on polystyrene plates were harvested, and the solubilized proteins were separated by 2-DE. When S. mutans biofilms were formed in the presence of S. gordonii, the peroxide resistance protein Dpr of the former showed 4.3-fold increased expression compared to biofilms that developed in the absence of the pioneer colonizer. In addition, we performed a competition assay using S. mutans antioxidant protein mutants together with S. gordonii and other initial colonizers. Growth of the dpr-knockout S. mutans mutant was significantly inhibited by S. gordonii, as well as by S. sanguinis. Furthermore, a cell viability assay revealed that the viability of the dpr-defective mutant was significantly attenuated compared to the wild-type strain when co-cultured with S. gordonii. Therefore, these results suggest that Dpr might be one of the essential proteins for S. mutans survival on teeth in the presence of early colonizing oral streptococci.
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影响因子：
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作者：
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通讯作者：
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