Mechanosensitive hair cell-like cells from embryonic and induced pluripotent stem cells.
Mechanosensitive hair cell-like cells from embryonic and induced pluripotent stem cells.
复制标题
DOI：
10.1016/j.cell.2010.03.035
复制
发表时间：
2010-05-14
期刊：
影响因子：
64.5
通讯作者：
Heller S
中科院分区：
文献类型：
--
作者：
Oshima K;Shin K;Diensthuber M;Peng AW;Ricci AJ;Heller S
机械敏感的感觉毛细胞是我们听觉和平衡感的关键。哺乳动物内耳无法再生失去的毛细胞是听力损失和某些平衡障碍永久性的主要原因。在这里，我们提出了一个逐步的指导协议开始与小鼠胚胎干细胞（ES）和诱导多能干细胞（iPS），这是针对成为外胚层能够响应耳诱导生长因子。将所得的耳祖细胞进行不同的分化条件，其中之一促进组织的细胞成上皮细胞簇显示毛细胞样细胞与静纤毛束。这些簇中的成束细胞对机械刺激产生的电流使人想起未成熟的毛细胞转导电流。
Mechanosensitive sensory hair cells are the linchpin of our senses of hearing and balance. The inability of the mammalian inner ear to regenerate lost hair cells is the major reason for the permanence of hearing loss and certain balance disorders. Here we present a stepwise guidance protocol starting with mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells, which were directed toward becoming ectoderm capable of responding to otic-inducing growth factors. The resulting otic progenitor cells were subjected to varying differentiation conditions, one of which promoted the organization of the cells into epithelial clusters displaying hair cell-like cells with stereociliary bundles. Bundle-bearing cells in these clusters responded to mechanical stimulation with currents that were reminiscent of immature hair cell transduction currents.
登录
查看更多内容
影响因子：
64.8
作者：
Glinka, A;Wu, W;Niehrs, C
通讯作者：
Niehrs, C
影响因子：
2.5
作者：
Lelli, Andrea;Asai, Yukako;Geleoc, Gwenaeelle S. G.
通讯作者：
Geleoc, Gwenaeelle S. G.
影响因子：
2.7
作者：
Alsina, B;Abelló, G;Giraldez, F
通讯作者：
Giraldez, F
影响因子：
3.6
作者：
Jinnin, M;Ihn, H;Tamaki, K
通讯作者：
Tamaki, K
影响因子：
25
作者：
Géléoc, GSG;Holt, JR
通讯作者：
Holt, JR