The δ- and ε-chains of the human T3/T-cell receptor complex are distinct polypeptides
The δ- and ε-chains of the human T3/T-cell receptor complex are distinct polypeptides
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人类 T3/T 细胞受体复合物的 δ 链和 ε 链是不同的多肽
作者：
J. Borst;J. Coligan;H. Oettgen;S. Pessano;R. Malin;C. Terhorst
人胸腺衍生淋巴细胞表面的T3/T细胞受体复合物由四种糖蛋白组成：相对分子质量（Mr）为40，000 - 50，000（40- 50 K）的α链、β链（37- 45 K）、γ链（25 K）和δ链（20 K）1-3。T3 α-和β-链已被鉴定为克隆型T细胞受体3 -7，但在功能上T3/T细胞受体链似乎形成单一复合物：针对20 K T3组分的单克隆抗体对正常人T淋巴细胞具有促有丝分裂作用8，9，在较高浓度下，抗克隆型和抗20 K试剂阻断T细胞功能4，10。最近，Zanders等人11表明，用高浓度抗原孵育人辅助性T细胞克隆可消除抗原特异性增殖，并诱导T3从细胞表面消失。因此，T3/T细胞受体复合物由两个可变亚基组成，T3 α链和β链，它们与抗原和单态20 K/25 K T3链相互作用。最近，第五条多肽链，即非糖基化的T3 ε-链的存在已被假定12，13。在这里，我们证实了20 K ε-链的存在。通过N-末端氨基酸测序、肽图谱和抗T3-δ和抗T3-ε抗体的免疫印迹判断，T3 ε链在一级结构上不同于T3 δ链。用内切糖苷酶F处理显示两条具有相同氨基末端的非糖基化T3 δ多肽主链（16 K和14 K）。与先前的脉冲追踪实验2一起，该观察结果表明，14 K T3多肽是通过分子C末端附近的蛋白水解加工从16 K T3前体衍生而来的。
The T3/T-cell receptor complex on the surface of human thymus-derived lymphocytes consists of four glycoproteins: the α-chain of relative molecular mass (Mr) 40,000–50,000 (40–50K), the β-chain (37–45K); the γ-chain (25K) and the δ-chain (20K)1–3. The T3 α- and β-chains have been identified as clonotypic T-cell receptors3–7, but functionally the T3/T-cell receptor chains seem to form a single complex: monoclonal antibodies directed at the 20K T3 components are mitogenic for normal human T lymphocytes8,9 and, at higher concentrations, anti-clonotypic and anti-20K reagents block T-cell function4,10. Recently, Zanders et al.11 showed that incubation of human T-helper clones with high concentrations of antigen abolishes antigen-specific proliferation and induces disappearance of T3 from the cell surface. Thus, the T3/T-cell receptor complex consists of two variable subunits, the T3 α- and β-chains, which interact with antigen and the monomorphic 20K/25K T3 chains. Recently, the existence of a fifth polypeptide chain, the unglycosylated T3 ε-chain, has been postulated12,13. Here we confirm that a 20K ε-chain does exist. The T3 ε-chain differs from the T3 δ-chain in primary structure as judged by N-terminal amino acid sequencing, peptide mapping and immuno-blotting with anti-T3-δ and anti-T3-ε antibodies. Treatment with endoglycosidase F revealed two nonglycosylated T3 δ polypeptide backbone chains (16K and 14K) with identical amino termini. Together with previous pulse-chase experiments2 this observation suggests that the 14K T3 polypeptide is derived from the 16K T3 precursor by proteolytic processing near the C-terminus of the molecule.
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人T细胞表面抗原T8的纯化和N端氨基酸序列。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Snow,PM;Keizer,G;Coligan,JE;Terhorst,C
通讯作者：
Terhorst,C
人类 T 细胞克隆个体上 T3 相关 49 道尔顿和 43 道尔顿细胞表面分子的比较：T 细胞受体结构中肽变异性的证据。
DOI：
10.1073/pnas.80.13.4104
发表时间：
1983
影响因子：
11.1
作者：
Reinherz,EL;Meuer,SC;Fitzgerald,KA;Hussey,RE;Hodgdon,JC;Acuto,O;Schlossman,SF
通讯作者：
Schlossman,SF
DOI：
10.1016/s0021-9258(18)32549-3
发表时间：
1983-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jannie Borst;Stephen Alexander;John Elder;Cox TerhorstSn
通讯作者：
Jannie Borst;Stephen Alexander;John Elder;Cox TerhorstSn
意大利面叠加：通过免疫放射自显影同时筛选多种多克隆和单克隆抗体。
影响因子：
2.9
作者：
Adair,WS
通讯作者：
Adair,WS
影响因子：
4.4
作者：
James P. Allison;Bradley W. McIntyre;D. Bloch
通讯作者：
James P. Allison;Bradley W. McIntyre;D. Bloch