Effects of intense magnetic fields on sedimentation pattern and gene expression profile in budding yeast
Effects of intense magnetic fields on sedimentation pattern and gene expression profile in budding yeast
复制标题
强磁场对芽殖酵母沉降模式和基因表达谱的影响
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
T. Koana
中科院分区：
文献类型：
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作者：
M. Ikehata;M. Iwasaka;J. Miyakoshi;S. Ueno;T. Koana
磁场对生物系统的影响通常使用生物学指标如基因表达谱来研究。然而，为了准确地评估MF的生物效应，必须认真考虑强MF对系统的物质传输过程，包括实验环境的影响。在这项研究中，培养的芽殖酵母，酿酒酵母，被用作体外生物测试系统的模型。暴露于5 T静态垂直MF后，我们发现细胞的沉降模式取决于培养皿在磁体孔中的位置。当将沉淀的细胞放置在磁体孔的下部时，它们位于培养皿的中心，而由于抗磁力，在放置在孔的上部的培养皿中细胞的沉淀是均匀的。暴露于5 T静态MF 2 h后酵母细胞的全基因组基因表达谱表明，MF不影响酵母细胞中任何基因的表达水平，尽管沉降模式改变。另外，10 T照射1 h和5 T照射24 h也不影响基因表达。另一方面，通过暴露于14 T静态MF 24 h，观察到与呼吸相关的几个基因的表达的轻微变化。本文还讨论了在体外研究微纤维的生物学效应时，估计微纤维间接效应的必要性。
Effects of magnetic fields (MFs) on biological systems are usually investigated using biological indices such as gene expression profiles. However, to precisely evaluate the biological effects of MF, the effects of intense MFs on systematic material transport processes including experimental environment must be seriously taken into consideration. In this study, a culture of the budding yeast, Saccharomyces cerevisiae, was used as a model for an in vitro biological test system. After exposure to 5 T static vertical MF, we found a difference in the sedimentation pattern of cells depending on the location of the dish in the magnet bore. Sedimented cells were localized in the center of the dish when they were placed in the lower part of the magnet bore while the sedimentation of the cells was uniform in dishes placed in the upper part of the bore because of the diamagnetic force. Genome wide gene expression profile of the yeast cells after exposure to 5 T static MF for 2 h suggested that the MF did not affect the expression level of any gene in yeast cells although the sedimentation pattern was altered. In addition, exposure to 10 T for 1 h and 5 T for 24 h also did not affect the gene expression. On the other hand, a slight change in expressions of several genes which are related to respiration was observed by exposure to a 14 T static MF for 24 h. The necessity of estimating the indirect effects of MFs on a study of its biological effect of MF in vitro will be discussed.
影响因子：
56.9
作者：
DeRisi, JL;Iyer, VR;Brown, PO
通讯作者：
Brown, PO
影响因子：
3.3
作者：
Gasch, AP;Spellman, PT;Brown, PO
通讯作者：
Brown, PO