Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT).
Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT).
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DOI：
10.1016/j.jcyt.2013.02.006
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发表时间：
2013-06
期刊：
影响因子：
4.5
通讯作者：
Gimble JM
中科院分区：
文献类型：
--
作者：
Bourin P;Bunnell BA;Casteilla L;Dominici M;Katz AJ;March KL;Redl H;Rubin JP;Yoshimura K;Gimble JM
脂肪组织是再生医学治疗方法的丰富和非常方便的细胞来源。然而，具有最大治疗潜力的脂肪基质干细胞(ASCs)群体的特征仍不清楚。在国际脂肪治疗联合会和国际细胞治疗学会的授权下，本文试图将基质细胞定义为未培养的基质血管分数(SVF)和贴壁基质/干细胞群体。鉴定脂肪来源细胞的表型和功能标准取自文献。在SVF中，细胞通过下列标志物进行表型识别：CD45-CD235a-CD31-CD34+。活性标记物和CD13、CD73、CD90和CD105这四种表面抗原均可提供附加值。成纤维细胞集落形成单位试验可以评估SVF人群中的祖细胞频率。在培养中，ASCs保留与其他间充质基质/干细胞(MSCs)相同的标记，包括CD90、CD73、CD105和CD44，而CD45和CD31为阴性。CD36阳性和CD106阴性可区别于骨髓间充质干细胞。建议用CFU-F法计算ASCs的群体倍增能力。脂肪细胞、软骨母细胞和成骨细胞分化试验用于完成细胞鉴定和潜能评估，同时通过生化或逆转录聚合酶链式反应对分化进行定量评估。这篇论文的目的是为从事脂肪细胞研究的科学界提供初步指导，并促进基于可重复参数的国际标准的发展。
Adipose tissue is a rich and very convenient source of cells for regenerative medicine therapeutic approaches. However, a characterization of the population of adipose-derived stromal and stem cells (ASCs) with the greatest therapeutic potential remains unclear. Under the authority of International Federation of Adipose Therapeutics and International Society for Cellular Therapy, this paper sets out to establish minimal definitions of stromal cells both as uncultured stromal vascular fraction (SVF) and as an adherent stromal/stem cells population. Phenotypic and functional criteria for the identification of adipose-derived cells were drawn from the literature. In the SVF, cells are identified phenotypically by the following markers: CD45-CD235a-CD31-CD34+. Added value may be provided by both a viability marker and the following surface antigens: CD13, CD73, CD90 and CD105. The fibroblastoid colony-forming unit assay permits the evaluation of progenitor frequency in the SVF population. In culture, ASCs retain markers in common with other mesenchymal stromal/stem cells (MSCs), including CD90, CD73, CD105, and CD44 and remain negative for CD45 and CD31. They can be distinguished from bone-marrow-derived MSCs by their positivity for CD36 and negativity for CD106. The CFU-F assay is recommended to calculate population doublings capacity of ASCs. The adipocytic, chondroblastic and osteoblastic differentiation assays serve to complete the cell identification and potency assessment in conjunction with a quantitative evaluation of the differentiation either biochemically or by reverse transcription polymerase chain reaction. The goal of this paper is to provide initial guidance for the scientific community working with adipose-derived cells and to facilitate development of international standards based on reproducible parameters.
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影响因子：
4.9
作者：
Maumus, M.;Peyrafitte, J-A;D'Angelo, R.;Fournier-Wirth, C.;Bouloumie, A.;Casteilla, L.;Sengenes, C.;Bourin, P.
通讯作者：
Bourin, P.
影响因子：
3.6
作者：
Li H;Zimmerlin L;Marra KG;Donnenberg VS;Donnenberg AD;Rubin JP
通讯作者：
Rubin JP
影响因子：
7.4
作者：
Astori, Giuseppe;Vignati, Francesca;Moccetti, Tiziano
通讯作者：
Moccetti, Tiziano
影响因子：
4.1
作者：
Casteilla, Louis;Planat-Benard, Valerie;Cousin, Beatrice
通讯作者：
Cousin, Beatrice
影响因子：
5.2
作者：
Mitchell, James B.;McIntosh, Kevin;Gimble, Jeffrey M.
通讯作者：
Gimble, Jeffrey M.