Native human adipose stromal cells: localization, morphology and phenotype.
Native human adipose stromal cells: localization, morphology and phenotype.
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DOI：
10.1038/ijo.2010.269
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发表时间：
2011-09
影响因子：
4.9
通讯作者：
Bourin, P.
中科院分区：
文献类型：
--
作者：
Maumus, M.;Peyrafitte, J-A;D'Angelo, R.;Fournier-Wirth, C.;Bouloumie, A.;Casteilla, L.;Sengenes, C.;Bourin, P.
除了在能量稳态和内分泌调节中发挥作用外，脂肪组织（AT）现在被认为是再生医学间充质基质细胞（脂肪源性基质细胞或ASC）的有前途的来源。尽管对培养的ASCs进行了大量研究，但很少对天然人ASCs进行研究。事实上，ASCs在其天然状态下的表型、其在AT内的定位以及与骨髓来源的间充质基质细胞（BM-MSC）的比较研究得很差。为了解决这些问题，提取人AT的基质血管部分（SVF），并通过免疫选择分离天然细胞亚型，以研究其在培养中的克隆形成潜力。对人AT样品进行免疫组织学分析，结合共聚焦切片重建，以定位ASCs。与BM-MNCs相比，所有天然ASCs均存在于AT-SVF的CD 34+细胞部分中。天然ASC表达针对BM-MSC描述的经典间充质标志物。有趣的是，在ASC细胞培养过程中，CD 34表达下降，并与细胞增殖率呈负相关。免疫组化分析显示，天然ASCs表现出特定的形态学特征与突起。发现它们分散在AT基质中，并且不表达在培养过程中出现的体内周细胞标记物，如NG 2、CD 140 b或α-平滑肌肌动蛋白。最后，在肥胖人群的AT中，ASCs自发向脂肪细胞谱系转化的能力增强。使用互补的方法学方法来研究天然人类ASC，揭示了它们的免疫表型、它们的特定形态、它们在AT内的位置和它们的干性。此外，我们的数据有力地表明，人类ASCs参与AT发展过程中的脂肪形成。
Beside having roles in energy homeostasis and endocrine modulation, adipose tissue (AT) is now considered a promising source of mesenchymal stromal cells (adipose-derived stromal cells or ASCs) for regenerative medicine. Despite numerous studies on cultured ASCs, native human ASCs are rarely investigated. Indeed, the phenotype of ASCs in their native state, their localization within AT and comparison with bone marrow-derived mesenchymal stromal cells (BM-MSCs) has been poorly investigated. To address these issues, the stroma vascular fraction (SVF) of human AT was extracted and native cell subtypes were isolated by immunoselection to study their clonogenic potential in culture. Immunohistology on samples of human AT in combination with reconstruction of confocal sections were performed in order to localize ASCs. Compared with BM-MNCs, all native ASCs were found in the CD34+ cell fraction of the AT-SVF. Native ASCs expressed classical mesenchymal markers described for BM-MSCs. Interestingly, CD34 expression decreased during ASC cell culture and was negatively correlated with cell proliferation rate. Immunohistological analysis revealed that native ASCs exhibited specific morphological features with protrusions. They were found scattered in AT stroma and did not express in vivo pericytic markers such as NG2, CD140b or alpha-smooth muscle actin, which appeared during the culture process. Finally, ASCs spontaneous commitment to adipocytic lineage was enhanced in AT from obese humans. The use of complementary methodological approaches to study native human ASCs revealed their immunophenotype, their specific morphology, their location within AT and their stemness. Furthermore, our data strongly suggest that human ASCs participate in adipogenesis during AT development.
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影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
影响因子：
4.5
作者：
Kaiser, S.;Hackanson, B.;Kapp, U.
通讯作者：
Kapp, U.
影响因子：
3.3
作者：
Boquest, AC;Shahdadfar, A;Brinchmann, JE
通讯作者：
Brinchmann, JE
影响因子：
5.2
作者：
Kern, Susanne;Eichler, Hermann;Bieback, Karen
通讯作者：
Bieback, Karen
影响因子：
5.2
作者：
Chen, JL;Hunt, P;Choi, ESH
通讯作者：
Choi, ESH