Pathogenic simian immunodeficiency virus infection is associated with expansion of the enteric virome.
Pathogenic simian immunodeficiency virus infection is associated with expansion of the enteric virome.
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DOI：
10.1016/j.cell.2012.09.024
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发表时间：
2012-10-12
期刊：
影响因子：
64.5
通讯作者：
Virgin HW
中科院分区：
文献类型：
--
作者：
Handley SA;Thackray LB;Zhao G;Presti R;Miller AD;Droit L;Abbink P;Maxfield LF;Kambal A;Duan E;Stanley K;Kramer J;Macri SC;Permar SR;Schmitz JE;Mansfield K;Brenchley JM;Veazey RS;Stappenbeck TS;Wang D;Barouch DH;Virgin HW
致病性猴免疫缺陷病毒(SIV)感染与肠道疾病有关，肠道疾病可能导致艾滋病的进展。为了确定艾滋病肠病的候选病因，我们使用下一代测序来确定非人类灵长类动物SIV感染期间的肠道病毒。致病的，但不是非致病的，SIV感染与肠道病毒的显著扩张有关。在致病性SIV感染期间，我们鉴定了至少32种以前未描述的肠道病毒，并通过病毒培养和聚合酶链式反应检测确认了它们的存在。在患有晚期艾滋病的动物中，我们检测到与肠炎相关的意外粘膜腺病毒感染以及微小病毒血症，表明SIV相关肠道病毒扩张的致病潜力。未发现致病性SIV感染与肠道细菌家族分类之间的关联。因此，肠道病毒感染可能导致艾滋病肠病和疾病进展。这些发现强调了对病毒的元基因组分析对于理解艾滋病发病机制的重要性。
Pathogenic simian immunodeficiency virus (SIV) infection is associated with enteropathy which likely contributes to AIDS progression. To identify candidate etiologies for AIDS enteropathy, we used next generation sequencing to define the enteric virome during SIV infection in nonhuman primates. Pathogenic, but not non-pathogenic, SIV infection was associated with significant expansion of the enteric virome. We identified at least 32 previously undescribed enteric viruses during pathogenic SIV infection and confirmed their presence using viral culture and PCR testing. We detected unsuspected mucosal adenovirus infection associated with enteritis as well as parvovirus viremia in animals with advanced AIDS, indicating the pathogenic potential of SIV-associated expansion of the enteric virome. No association between pathogenic SIV infection and the family-level taxonomy of enteric bacteria was detected. Thus, enteric viral infections may contribute to AIDS enteropathy and disease progression. These findings underline the importance of metagenomic analysis of the virome for understanding AIDS pathogenesis.
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影响因子：
5.4
作者：
Farkas, Tibor;Sestak, Karol;Jiang, Xi
通讯作者：
Jiang, Xi
影响因子：
32.4
作者：
Brenchley JM;Silvestri G;Douek DC
通讯作者：
Douek DC
影响因子：
64.5
作者：
Cadwell K;Patel KK;Maloney NS;Liu TC;Ng AC;Storer CE;Head RD;Xavier R;Stappenbeck TS;Virgin HW
通讯作者：
Virgin HW
DOI：
10.1126/science.1221789
发表时间：
2012-06-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Iliev ID;Funari VA;Taylor KD;Nguyen Q;Reyes CN;Strom SP;Brown J;Becker CA;Fleshner PR;Dubinsky M;Rotter JI;Wang HL;McGovern DP;Brown GD;Underhill DM
通讯作者：
Underhill DM
影响因子：
3
作者：
Huson DH;Richter DC;Mitra S;Auch AF;Schuster SC
通讯作者：
Schuster SC