Comparative genome analysis of 12 Shigella sonnei strains: virulence, resistance, and their interactions
Comparative genome analysis of 12 Shigella sonnei strains: virulence, resistance, and their interactions
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12 种宋氏志贺菌的比较基因组分析：毒力、耐药性及其相互作用
DOI：
10.1007/s10123-020-00145-x
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发表时间：
2019-02
影响因子：
3.1
通讯作者：
Bing Gu
中科院分区：
文献类型：
--
作者：
Zuobin Zhu;Liang Wang;Huimin Qian;Feng Gu;Ying Li;Heng Zhang;Ying Chen;Jiajia Shi;Ping Ma;Changjun Bao;Bing Gu
志贺氏菌病是一种高度传染性疾病，主要通过粪便-口腔接触志贺氏菌传播。志贺菌属目前已鉴定出4个种，其中福氏志贺菌是全球最流行的种，且多在发展中国家分离。然而，它正在被宋内志贺菌所取代，宋内志贺菌目前是亚洲和中东等许多新兴工业化国家痢疾大流行的主要病原体。为了更好地理解S.对12株临床宋内氏志贺菌的毒力和耐药性进行了全序列测定。从江苏省4个城市采集的宋内氏菌株具有不同的抗药性。系统基因组学分析将敏感和抗性S. sonnei分为两个不同的群体，而泛基因组分析揭示了每个群体中存在和不存在独特的基因。通过对31类毒力因子的筛选，发现耐药菌株的2型分泌系统加倍。基于毒力和抗性之间相互作用的进一步主成分分析表明，丰富的毒力因子与较高水平的抗生素抗性相关。本文的结果是基于小样本的统计分析，并基本上作为进一步的实验和理论研究的指导。
Shigellosis is a highly infectious disease that is mainly transmitted via fecal-oral contact of the bacteria Shigella. Four species have been identified in Shigella genus, among which Shigella flexneri is used to be the most prevalent species globally and commonly isolated from developing countries. However, it is being replaced by Shigella sonnei that is currently the main causative agent for dysentery pandemic in many emerging industrialized countries such as Asia and the Middle East. For a better understanding of S. sonnei virulence and antibiotic resistance, we sequenced 12 clinical S. sonnei strains with varied antibiotic-resistance profiles collected from four cities in Jiangsu Province, China. Phylogenomic analysis clustered antibiotic-sensitive and resistant S. sonnei into two distinct groups while pan-genome analysis reveals the presence and absence of unique genes in each group. Screening of 31 classes of virulence factors found out that type 2 secretion system is doubled in resistant strains. Further principle component analysis based on the interactions between virulence and resistance indicated that abundant virulence factors are associated with higher levels of antibiotic resistance. The result present here is based on statistical analysis of a small sample size and serves basically as a guidance for further experimental and theoretical studies.
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影响因子：
4.4
作者：
Watson J;Jenkins C;Clements A
通讯作者：
Clements A
影响因子：
10.7
作者：
Price MN;Dehal PS;Arkin AP
通讯作者：
Arkin AP
志贺氏菌种类的基因组动力学和多样性，杆菌痢疾的病因学剂。
影响因子：
14.9
作者：
Yang, F;Yang, J;Zhang, XB;Chen, LH;Jiang, Y;Yan, YL;Tang, XD;Wang, J;Xiong, ZH;Dong, J;Xue, Y;Zhu, YF;Xu, XY;Sun, LL;Chen, SX;Nie, H;Peng, JP;Xu, JG;Wang, Y;Yuan, ZH;Wen, YM;Yao, ZJ;Shen, Y;Qiang, BQ;Hou, YD;Yu, J;Jin, Q
通讯作者：
Jin, Q
影响因子：
7
作者：
Li, Ruiqiang;Zhu, Hongmei;Wang, Jun
通讯作者：
Wang, Jun
影响因子：
5.2
作者：
Cameron, David R.;Mortin, Lawrence I.;Peleg, Anton Y.
通讯作者：
Peleg, Anton Y.