Protein-engineered biomaterials: nanoscale mimics of the extracellular matrix.
Protein-engineered biomaterials: nanoscale mimics of the extracellular matrix.
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DOI：
10.1016/j.bbagen.2010.07.005
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发表时间：
2011-03
影响因子：
3
通讯作者：
Heilshorn, Sarah C.
中科院分区：
文献类型：
--
作者：
Romano, Nicole H.;Sengupta, Debanti;Chung, Cindy;Heilshorn, Sarah C.
关键词：
用作体外细胞培养基质的传统材料是刚性和平坦的表面，其缺乏体内细胞外环境的精细纳米和微米尺度特征。虽然这些表面可以用收获的细胞外基质（ECM）蛋白包被以部分概括ECM的生物指导性质，但这些收获的蛋白质通常表现出大的批次间变异性，并且可能难以定制用于特定的生物学研究。相比之下，重组蛋白技术可用于合成细胞相容性、可重现性和完全可定制的蛋白质工程生物材料家族。在这里，我们描述了一种模块化的设计策略，以合成蛋白质工程生物材料，融合在一起的纳米级肽设计基序的多个重复到全长工程ECM模拟物。由于重组蛋白质合成的分子水平的精度，这些生物材料可以被定制为包括在指定密度的各种生物指导配体，以表现出与天然组织的机械性能相匹配的机械性能，并包括能够实现细胞触发的支架重塑的蛋白水解靶位点。此外，这些生物材料可以加工成可注射的形式，用于微创递送或空间图案化，以使多种药物能够以不同的释放动力学释放。鉴于这些蛋白质工程生物材料的可重复性和灵活性，它们是细胞-基质相互作用的还原生物学研究、生理过程的体外模型和再生医学疗法中的生物指导性支架的理想基质。
Traditional materials used as in vitro cell culture substrates are rigid and flat surfaces that lack the exquisite nano- and micro-scale features of the in vivo extracellular environment. While these surfaces can be coated with harvested extracellular matrix (ECM) proteins to partially recapitulate the bio-instructive nature of the ECM, these harvested proteins often exhibit large batch-to-batch variability and can be difficult to customize for specific biological studies. In contrast, recombinant protein technology can be utilized to synthesize families of protein-engineered biomaterials that are cyto-compatible, reproducible, and fully customizable. Here we describe a modular design strategy to synthesize protein-engineered biomaterials that fuse together multiple repeats of nanoscale peptide design motifs into full-length engineered ECM mimetics. Due to the molecular-level precision of recombinant protein synthesis, these biomaterials can be tailored to include a variety of bio-instructional ligands at specified densities, to exhibit mechanical properties that match those of native tissue, and to include proteolytic target sites that enable cell-triggered scaffold remodeling. Furthermore, these biomaterials can be processed into forms that are injectable for minimally-invasive delivery or spatially patterned to enable the release of multiple drugs with distinct release kinetics. Given the reproducibility and flexibility of these protein-engineered biomaterials, they are ideal substrates for reductionist biological studies of cell-matrix interactions, for in vitro models of physiological processes, and for bio-instructive scaffolds in regenerative medicine therapies.
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DOI：
10.1016/sasj-2006-0007-rr
发表时间：
2007
期刊：
SAS journal
影响因子：
--
作者：
Bao, Qi-Bin;Songer, Matthew;Pimenta, Luis;Werner, Dieter;Reyes-Sanchez, Alejandro;Balsano, Massimo;Agrillo, Umberto;Coric, Domagoj;Davenport, Kenneth;Yuan, Hansen
通讯作者：
Yuan, Hansen
影响因子：
56.9
作者：
Cukierman, E;Pankov, R;Yamada, KM
通讯作者：
Yamada, KM
影响因子：
2.9
作者：
Chow, Dominic C.;Dreher, Matthew R.;Chilkoti, Ashutosh
通讯作者：
Chilkoti, Ashutosh
影响因子：
14
作者：
Asakura, T;Tanaka, C;Kurokawa, M
通讯作者：
Kurokawa, M
影响因子：
4.8
作者：
Gobin, AS;West, JL
通讯作者：
West, JL