Comparative Genome Analysis of Bifidobacterium longum subsp. infantis Strains Reveals Variation in Human Milk Oligosaccharide Utilization Genes among Commercial Probiotics.
Comparative Genome Analysis of Bifidobacterium longum subsp. infantis Strains Reveals Variation in Human Milk Oligosaccharide Utilization Genes among Commercial Probiotics.
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DOI：
10.3390/nu12113247
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发表时间：
2020-10-23
期刊：
影响因子：
5.9
通讯作者：
Frese SA
中科院分区：
文献类型：
--
作者：
Duar RM;Casaburi G;Mitchell RD;Scofield LNC;Ortega Ramirez CA;Barile D;Henrick BM;Frese SA
生态失调与新生儿的急性和长期后果有关。益生菌可以有效地限制与生态失调相关的细菌的生长，并促进婴儿微生物组的健康发育。鉴于其适应婴儿肠道，并从动物和体外模型的有希望的数据，长双歧杆菌亚种。益生菌是用于婴儿益生菌的有吸引力的候选者。然而，商业化菌株利用人乳低聚糖（HMO）能力的菌株水平差异可能会影响菌株在婴儿肠道中的性能。在这项研究中，我们表征了12个B。的益生菌菌株，并确定了两个主要的变种之一的HMO利用基因簇。一些菌株拥有HMO利用基因的全部库（H5阳性菌株），而H5阴性菌株缺乏已知结合核心HMO结构的ABC型转运蛋白。H5-阳性菌株在乳糖-N-四糖和乳糖-N-新四糖上实现了显著的上级生长。在体外，H5阳性菌株有一个显着的健身优势H5阴性菌株，这也是在母乳喂养的婴儿体内观察。这项工作为B之间遗传差异的功能影响提供了证据。的菌株，并强调，基因型和HMO利用表型时，应考虑选择菌株的益生菌用于婴儿。
Dysbiosis is associated with acute and long-term consequences for neonates. Probiotics can be effective in limiting the growth of bacteria associated with dysbiosis and promoting the healthy development of the infant microbiome. Given its adaptation to the infant gut, and promising data from animal and in vitro models, Bifidobacterium longum subsp. infantis is an attractive candidate for use in infant probiotics. However, strain-level differences in the ability of commercialized strains to utilize human milk oligosaccharides (HMOs) may have implications in the performance of strains in the infant gut. In this study, we characterized twelve B. infantis probiotic strains and identified two main variants in one of the HMO utilization gene clusters. Some strains possessed the full repertoire of HMO utilization genes (H5-positive strains), while H5-negative strains lack an ABC-type transporter known to bind core HMO structures. H5-positive strains achieved significantly superior growth on lacto-N-tetraose and lacto-N-neotetraose. In vitro, H5-positive strains had a significant fitness advantage over H5-negative strains, which was also observed in vivo in breastfed infants. This work provides evidence of the functional implications of genetic differences among B. infantis strains and highlights that genotype and HMO utilization phenotype should be considered when selecting a strain for probiotic use in infants.
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影响因子：
4.4
作者：
Cheng, Christopher C.;Duar, Rebbeca;Walter, Jens
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
Henrick BM;Hutton AA;Palumbo MC;Casaburi G;Mitchell RD;Underwood MA;Smilowitz JT;Frese SA
通讯作者：
Frese SA
影响因子：
1.7
作者：
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通讯作者：
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