Plasmalemmal and vesicular gamma-aminobutyric acid transporter expression in the developing mouse retina.
Plasmalemmal and vesicular gamma-aminobutyric acid transporter expression in the developing mouse retina.
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DOI：
10.1002/cne.21846
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发表时间：
2009-01-01
影响因子：
2.5
通讯作者：
Brecha, Nicholas C.
中科院分区：
文献类型：
--
作者：
Guo, Chenying;Stella, Salvatore L., Jr.;Hirano, Arlene A.;Brecha, Nicholas C.
质膜和囊泡γ-氨基丁酸（GABA）转运蛋白通过调节高亲和力GABA摄取和GABA释放到突触间隙和细胞外间隙来影响神经传递。出生后表达的质膜GABA转运蛋白-1（GAT-1），GAT-3，和囊泡GABA/甘氨酸转运蛋白（VGAT）在发育中的小鼠视网膜进行了评价，通过使用免疫组化与亲和纯化的抗体。在出生后0天，在视网膜内层观察到弱的转运蛋白免疫反应（P0）。GAT-1免疫染色在P0和在老年分别在内核层（INL）和神经节细胞层（GCL）的无长突细胞和移位无长突细胞中，以及在内丛状层（IPL）的突起中。P10时Müller细胞突起GAT-1免疫染色较弱。GAT-3免疫染色在P0和更老的年龄是在无长突细胞和他们的过程，以及在米勒细胞和他们的过程，径向延伸整个视网膜。P10时，Müller细胞胞体位于INL中部。VGAT免疫染色存在于P0和年龄较大的INL的无长突细胞以及IPL的过程中。在P5，在水平细胞胞体和突起中也观察到弱的VGAT免疫染色。到P15，GAT和VGAT免疫染色模式似乎与成人免疫染色模式相似;它们在P20左右达到成人水平。这些研究结果表明，GABA的摄取和释放最初建立在视网膜内层在出生后的第一周，这些系统随后成熟的视网膜外层在出生后的第二周。
Plasmalemmal and vesicular γ-aminobutyric acid (GABA) transporters influence neurotransmission by regulating high-affinity GABA uptake and GABA release into the synaptic cleft and extracellular space. Postnatal expression of the plasmalemmal GABA transporter-1 (GAT-1), GAT-3, and the vesicular GABA/glycine transporter (VGAT) were evaluated in the developing mouse retina by using immunohistochemistry with affinity-purified antibodies. Weak transporter immunoreactivity was observed in the inner retina at postnatal day 0 (P0). GAT-1 immunostaining at P0 and at older ages was in amacrine and displaced amacrine cells in the inner nuclear layer (INL) and ganglion cell layer (GCL), respectively, and in their processes in the inner plexiform layer (IPL). At P10, weak GAT-1 immunostaining was in Müller cell processes. GAT-3 immunostaining at P0 and older ages was in amacrine cells and their processes, as well as in Müller cells and their processes that extended radially across the retina. At P10, Müller cell somata were observed in the middle of the INL. VGAT immunostaining was present at P0 and older ages in amacrine cells in the INL as well as processes in the IPL. At P5, weak VGAT immunostaining was also observed in horizontal cell somata and processes. By P15, the GAT and VGAT immunostaining patterns appear similar to the adult immunostaining patterns; they reached adult levels by about P20. These findings demonstrate that GABA uptake and release are initially established in the inner retina during the first postnatal week and that these systems subsequently mature in the outer retina during the second postnatal week.
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影响因子：
2.5
作者：
BRECHA, NC;WEIGMANN, C
通讯作者：
WEIGMANN, C
DOI：
10.1016/s0922-4106(05)80040-9
发表时间：
1991-05-25
期刊：
EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION
影响因子：
--
作者：
CHRISTENSEN, H;FYSKE, EM;FONNUM, F
通讯作者：
FONNUM, F
影响因子：
2.5
作者：
AGARDH, E;BRUUN, A;WU, JY
通讯作者：
WU, JY
影响因子：
3.3
作者：
AGARDH, E;EHINGER, B
通讯作者：
EHINGER, B
DOI：
10.1083/jcb.97.3.703
发表时间：
1983-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bunt-Milam AH;Saari JC
通讯作者：
Saari JC