Anatomy and transcript profiling of gynoecium development in female sterile Brassica napus mediated by one alien chromosome from Orychophragmus violaceus.
Anatomy and transcript profiling of gynoecium development in female sterile Brassica napus mediated by one alien chromosome from Orychophragmus violaceus.
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雌性无菌胸罩的妇科发育的解剖学和笔录分析，由Orychophragmus vileaceus介导的一个外星染色体介导。
DOI：
10.1186/1471-2164-15-61
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发表时间：
2014-01-23
期刊：
影响因子：
4.4
通讯作者：
Li ZY
中科院分区：
文献类型：
--
作者：
Fu WQ;Zhao ZG;Ge XH;Ding L;Li ZY
雌蕊群是被子植物中最复杂的种子生产和传播器官之一，但利用雌性不育突变体研究发现，雌蕊群中只有几个与胚珠或胚囊发育有关的基因。油菜雌性不育系的产生与来自十字花科蔬菜诸葛菜的一条外源染色体有关。在此，对雌性不育进行发育解剖学和比较转录谱分析（RNA-seq），以揭示受损雌蕊形成背后的基因和可能的代谢途径。甘蓝型油菜（Brassica napus）具有两个拷贝的外源染色体（S1）的雌性不育系的胚珠仅发育出一个短珠被原基，该原基不能进一步发育，雌配子体发育在四分体后、雌配子体发育开始前受阻。以Brassica_95 k_ unigene为参考基因组，在S1和供体B中分别鉴定出28,065和27,653个unigene。napus（H3）。进一步比较S1和H3之间的转录本丰度，发现4540个unigenes表现出超过两倍的表达差异。差异表达基因（DEGs）的基因本体和途径富集分析表明，许多重要基因和代谢途径参与了雌蕊群、胚囊、胚珠、珠被的发育以及花粉与雌蕊的相互作用。DEG在油菜素内酯（BR）合成、近轴/远轴分化、生长素运输和信号转导等代谢途径中发挥重要作用。提出了一个模型，以显示这些途径的不育雌蕊发育的可能作用和相互作用。这些结果为B雌蕊群早期发育的分子机制提供了新的信息。油菜。
The gynoecium is one of the most complex organs of angiosperms specialized for seed production and dispersal, but only several genes important for ovule or embryo sac development were identified by using female sterile mutants. The female sterility in oilseed rape (Brassica napus) was before found to be related with one alien chromosome from another crucifer Orychophragmus violaceus. Herein, the developmental anatomy and comparative transcript profiling (RNA-seq) for the female sterility were performed to reveal the genes and possible metabolic pathways behind the formation of the damaged gynoecium. The ovules in the female sterile Brassica napus with two copies of the alien chromosomes (S1) initiated only one short integument primordium which underwent no further development and the female gametophyte development was blocked after the tetrad stage but before megagametogenesis initiation. Using Brassica_ 95k_ unigene as the reference genome, a total of 28,065 and 27,653 unigenes were identified to be transcribed in S1 and donor B. napus (H3), respectively. Further comparison of the transcript abundance between S1 and H3 revealed that 4540 unigenes showed more than two fold expression differences. Gene ontology and pathway enrichment analysis of the Differentially Expressed Genes (DEGs) showed that a number of important genes and metabolism pathways were involved in the development of gynoecium, embryo sac, ovule, integuments as well as the interactions between pollen and pistil. DEGs for the ovule development were detected to function in the metabolism pathways regulating brassinosteroid (BR) biosynthesis, adaxial/abaxial axis specification, auxin transport and signaling. A model was proposed to show the possible roles and interactions of these pathways for the sterile gynoecium development. The results provided new information for the molecular mechanisms behind the gynoecium development at early stage in B. napus.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
Ferr치ndiz, C
通讯作者：
Ferr치ndiz, C