A Study on the Mechanism of Cinobufagin in the Treatment of Paw Cancer Pain by Modulating Local β -Endorphin Expression In Vivo.
A Study on the Mechanism of Cinobufagin in the Treatment of Paw Cancer Pain by Modulating Local β -Endorphin Expression In Vivo.
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华蟾素调节局部β-内啡肽体内表达治疗爪癌疼痛的机制研究
DOI：
10.1155/2013/851256
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Zhan L
中科院分区：
文献类型：
--
作者：
Chen T;Hu W;He H;Gong Z;Wang J;Yu X;Ai T;Zhan L
背景：华蟾毒精已广泛用于癌症治疗，在缓解癌痛方面发挥重要作用，但相关机制尚不清楚。
目的：利用爪部癌痛模型研究小鼠爪部癌痛中热痛觉过敏和机械痛觉过敏的变化以及华蟾毒精的作用机制。
方法：60只雌性小鼠随机分为5组：对照组、模型组、华蟾毒精组、华蟾毒精 + NAL - M组和吗啡组；除对照组外，小鼠右后爪接种H22肝癌细胞。接种后第9天起，小鼠每日给药1次，持续8天。在给药前后第2、4、6和8天测定疼痛行为。最后一天处死小鼠。采用酶联免疫吸附测定（ELISA）分析β - 内啡肽（β - END）、促肾上腺皮质激素释放因子（CRF）和白细胞介素 - 1β（IL - 1β）的水平；采用免疫组织化学法检测肿瘤及邻近组织中β - END、阿黑皮素原（POMC）和μ - 阿片受体（μ - OR）的表达。
结果：华蟾毒精显著提高热痛和机械痛阈值。此外，华蟾毒精显著上调β - END、CRF、POMC和μ - OR的表达。华蟾毒精的镇痛作用被外周阿片受体拮抗剂NAL - M阻断。
结论：华蟾毒精显著缓解小鼠癌痛并提高其痛阈值，主要是上调后爪肿瘤及邻近组织中β - END和μ - OR的表达水平。
Background. Cinobufagin has been widely used in the treatment of carcinoma and plays an important role in the relief of cancer pain. But the involved mechanism remains unknown. Aim. To investigate the changes in thermal and mechanical hyperalgesia in paw cancer pain in mice and the action mechanism of cinobufagin using a paw cancer pain model. Methods. 60 female mice were randomly divided into 5 groups: control group, model group, cinobufagin group, cinobufagin +NAL-M group, and morphine group; except ones in control group, mice were inoculated with H22 hepatoma cells in the right hind paw. From the 9th day after inoculation, mice were administrated drug once daily lasting for 8 days. The pain behavior was determined on the 2nd, 4th, 6th, and 8th days before and after administration. On the last day, they were sacrificed. The levels of β-END, CRF, and IL-1β were analyzed by ELISA; immunohistochemistry was performed to detect the expressions of β-END, POMC, and μ-OR in the tumor and adjacent tissue. Results. The thresholds of thermal pain and mechanical pain were significantly increased by cinobufagin. Moreover, the expressions of β-END, CRF, POMC, and μ-OR were significantly upregulated by cinobufagin. The analgesic effect of cinobufagin was blocked by the peripheral opioid receptor antagonist NAL-M. Conclusions. Cinobufagin significantly relieved cancer pain in mice and raised their pain threshold, mainly upregulating the expression levels of β-END and μ-OR in the hind paw tumor and adjacent tissue.
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影响因子：
3.7
作者：
Doré-Savard L;Otis V;Belleville K;Lemire M;Archambault M;Tremblay L;Beaudoin JF;Beaudet N;Lecomte R;Lepage M;Gendron L;Sarret P
通讯作者：
Sarret P
影响因子：
7.4
作者：
Medhurst, SJ;Walker, K;Urban, L
通讯作者：
Urban, L
影响因子：
3.6
作者：
Vermeirsch, H;Nuydens, RM;Meert, TF
通讯作者：
Meert, TF
影响因子：
3.6
作者：
El Mouedden, M;Meert, TF
通讯作者：
Meert, TF
用Tong-luo-san-jie凝胶局部治疗可减轻大鼠的骨癌疼痛。
影响因子：
5.4
作者：
Wang, Juyong;Zhang, Ruixin;Dong, Changsheng;Jiao, Liying;Xu, Ling;Liu, Jiyong;Wang, Zhengtao;Ying, Qi Liang Mao;Fong, Harry;Lao, Lixing
通讯作者：
Lao, Lixing