Topical treatment with Tong-Luo-San-Jie gel alleviates bone cancer pain in rats.
Topical treatment with Tong-Luo-San-Jie gel alleviates bone cancer pain in rats.
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用Tong-luo-san-jie凝胶局部治疗可减轻大鼠的骨癌疼痛。
DOI：
10.1016/j.jep.2012.08.026
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发表时间：
2012-10-11
影响因子：
5.4
通讯作者：
Lao, Lixing
中科院分区：
文献类型：
--
作者：
Wang, Juyong;Zhang, Ruixin;Dong, Changsheng;Jiao, Liying;Xu, Ling;Liu, Jiyong;Wang, Zhengtao;Ying, Qi Liang Mao;Fong, Harry;Lao, Lixing
关键词：
通络散结（TLSJ）中药止痛凝胶及其改良配方在中医中用于控制癌症疼痛，然而其作用机制仍不明确。
为研究TLSJ凝胶对大鼠骨癌疼痛的影响及其机制。
通过将Walker 256大鼠癌细胞直接接种到Sprague - Dawley大鼠（150 - 170克）的右胫骨骨髓腔建立骨癌疼痛大鼠模型；以磷酸盐缓冲盐水（PBS）胫骨接种作为对照。患癌大鼠每天两次外用TLSJ凝胶（0.5克/平方厘米/天）或惰性凝胶对照进行治疗，持续21天（每组n = 10）。进行了机械阈值和缩爪潜伏期（PWL）等行为学测试。治疗后测定破骨细胞活性，并通过酶联免疫吸附测定（ELISA）检测羧基末端吡啶啉交联的I型胶原末端肽（ICTP）和骨特异性碱性磷酸酶（BAP）浓度。监测不良反应，并对局部使用TLSJ凝胶治疗6周的未患癌大鼠进行生化和组织学检测。
与惰性凝胶相比，TLSJ治疗显著恢复了骨癌导致的PWL和机械阈值的降低。它还降低了血清ICTP和BAP水平，并抑制了破骨细胞活性。TLSJ治疗后未检测到不良反应或异常的生化和组织学变化。
本研究表明，TLSJ显著抑制骨癌诱导的热痛觉和机械痛觉过敏。这表明该凝胶可能有助于控制癌症疼痛，并且可能通过抑制破骨细胞活性发挥作用。
The herbal analgesic gel Tong-Luo-San-Jie (TLSJ) and its modifications are used in traditional Chinese medicine to manage cancer pain. However, its mechanisms are still unknown. To investigate the effects and mechanisms of TLSJ gel on bone cancer pain in a rat model. A bone cancer pain rat model was established by inoculating Walker 256 rat carcinoma cells directly into the right tibial medullary cavity of Sprague-Dawley rats (150–170 g); Phosphate buffered saline (PBS) tibial inoculation was used as control. Cancer-bearing rats were treated twice a day with external TLSJ gel (0.5 g/cm2/day) or inert gel control for 21 days (n=10/group). Behavioral tests such as mechanical threshold and paw withdrawal latency (PWL) were carried out. Osteoclastic activities were determined and carboxyterminal pyridinoline cross-linked type I collagen telopeptides (ICTP) and bone-specific alkaline phosphatase (BAP) concentrations were detected with ELISA after treatment. Adverse effects were monitored, and biochemical and histological tests were performed in naïve rats treated with local TLSJ gel for six weeks. TLSJ treatment significantly restored bone cancer-induced decrease of PWL and mechanical threshold compared to inert gel. It also decreased the level of blood serum ICTP and BAP and inhibited osteoclast activities. No adverse effects or abnormal biochemical and histological changes were detected after TLSJ treatment. The present study shows that TLSJ significantly inhibits bone cancer-induced thermal and mechanical sensitization. It suggests that the gel may be useful in managing cancer pain and that it may act by inhibiting osteoclastic activity.
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影响因子：
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