Specialized pro-resolving receptors are expressed in salivary glands with Sjögren's syndrome.
Specialized pro-resolving receptors are expressed in salivary glands with Sjögren's syndrome.
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DOI：
10.1016/j.anndiagpath.2021.151865
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发表时间：
2022-03
影响因子：
2
通讯作者：
Baker OJ
中科院分区：
文献类型：
--
作者：
Dos Santos HT;Nam K;Maslow F;Trump B;Baker OJ
我们之前的研究表明，在Sjögren综合征（SS）样小鼠模型发病前后，resolvin D1 （RvD1）及其阿斯匹林触发（AT）形式AT-RvD1可有效减少炎症，同时恢复唾液流速。溶解蛋白是专门的促溶解介质（SPM），积极调节炎症。然而，我们只在唾液腺中对RvD1和AT-RvD1有广泛的数据，它们都与受体ALX/FPR2结合。因此，唾液腺内其他SPM受体的存在是未知的。因此，本研究的目的是确定SPM受体在非SS和SS患者中的表达。为此，我们使用Chisholm和Mason分类对6个女性受试者的人小唾液腺进行H&E分析，以确定淋巴细胞浸润的程度。接下来，共聚焦免疫荧光分析确定不同SPM受体在粘液腺泡和纹状管中的存在和分布。我们观察到弥漫性淋巴细胞浸润，临床资料与SS诊断一致。此外，共聚焦免疫荧光分析显示，非SS和SS患者的粘膜腺泡和条纹管中均存在受体ALX/FPR2、BLT1和CMKLR1。GPR32在SS和非SS小唾液腺中均不存在。综上所述，我们的研究结果表明，多种SPM受体在非SS和SS小唾液腺中表达，所有这些受体都可能成为促进SS患者促上皮和抗炎/促溶解信号传导的潜在靶点。
Our previous studies demonstrated that resolvin D1 (RvD1) and its aspirin-trigged (AT) form AT-RvD1, are effective in decreasing inflammation while restoring saliva flow rates in a Sjögren’s syndrome (SS)-like mouse model before and after disease onset. Resolvins are specialized pro-resolving mediators (SPM) that actively regulate inflammation. However, we only have extensive data within the salivary glands for RvD1 and AT-RvD1, both of which bind to the receptor ALX/FPR2. As such, the presence of other SPM receptors is unknown within salivary glands. Therefore, the goal of this study was to determine the expression of SPM receptors in non-SS and SS patients. For this purpose, six human minor salivary glands from female subjects were analyzed by H&E using the Chisholm and Mason classification to determine the degree of lymphocytic infiltration. Next, confocal immunofluorescence analysis was performed to determine the presence and distribution of different SPM receptors in mucous acini and striated ducts. We observed diffuse presence of lymphocytic infiltration and clinical data were consistent with SS diagnosis in three patients. Moreover, confocal immunofluorescence analysis indicated the presence of the receptors ALX/FPR2, BLT1 and CMKLR1 in the mucous acini and striated ducts of both non-SS and SS patients. GPR32 was absent in SS and non-SS minor salivary glands. In summary, our results showed that various SPM receptors are expressed in non-SS and SS minor salivary glands, all of which may pose as potential targets for promoting pro-epithelial and anti-inflammatory/pro-resolution signaling on SS patients.
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