LINE-1 transcription in round spermatids is associated with accretion of 5-carboxylcytosine in their open reading frames.
LINE-1 transcription in round spermatids is associated with accretion of 5-carboxylcytosine in their open reading frames.
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DOI：
10.1038/s42003-021-02217-8
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发表时间：
2021-06-07
影响因子：
5.9
通讯作者：
Ruzov A
中科院分区：
文献类型：
--
作者：
Blythe MJ;Kocer A;Rubio-Roldan A;Giles T;Abakir A;Ialy-Radio C;Wheldon LM;Bereshchenko O;Bruscoli S;Kondrashov A;Drevet JR;Emes RD;Johnson AD;McCarrey JR;Gackowski D;Olinski R;Cocquet J;Garcia-Perez JL;Ruzov A
雄配子和雌配子的染色质在从生殖细胞到胚胎发育程序的转变过程中经历了一系列的重新编程事件。尽管在受精卵中发生的DNA甲基化模式的重排已经被广泛地描述，但对精子细胞成熟过程中DNA修饰的动态知之甚少。在这里，我们证明了5-羧基胞嘧啶(5caC)的动态与LINE-1逆转录元件在小鼠精子发生过程中的活跃转录有关。我们发现，活跃和进化的年轻LINE-1S的开放阅读框架在圆形精子细胞中富含5caC，而在精子细胞成熟过程中，LINE-1S和精子发生特异基因中的5CaC被消除，同时保留在发育基因的启动子和内含子中。我们的结果显示，5caC与LINE-1反转录转座子的活性有关，这表明这种DNA修饰在其转录的精细调控中具有潜在的直接作用。Blythe等人研究了精子发生过程中5-甲基胞嘧啶DNA修饰的氧化形式。他们发现，在精子细胞成熟过程中，进化上年轻且转录活性较高的LINE-1反转录转座子的开放阅读框架富含5-羧基胞嘧啶(5caC)，这表明5CaC与LINE-1活性之间存在联系。
Chromatin of male and female gametes undergoes a number of reprogramming events during the transition from germ cell to embryonic developmental programs. Although the rearrangement of DNA methylation patterns occurring in the zygote has been extensively characterized, little is known about the dynamics of DNA modifications during spermatid maturation. Here, we demonstrate that the dynamics of 5-carboxylcytosine (5caC) correlate with active transcription of LINE-1 retroelements during murine spermiogenesis. We show that the open reading frames of active and evolutionary young LINE-1s are 5caC-enriched in round spermatids and 5caC is eliminated from LINE-1s and spermiogenesis-specific genes during spermatid maturation, being simultaneously retained at promoters and introns of developmental genes. Our results reveal an association of 5caC with activity of LINE-1 retrotransposons suggesting a potential direct role for this DNA modification in fine regulation of their transcription. Blythe et al investigate the patterns of oxidized forms of the 5-methylcytosine DNA modification during spermatogenesis. They find that open reading frames of evolutionarily young and transcriptionally active LINE-1 retrotransposons are enriched in 5-carboxylcytosine (5caC) during spermatid maturation, suggesting a link between 5cac and LINE-1 activity.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
11.8
作者：
Green CD;Ma Q;Manske GL;Shami AN;Zheng X;Marini S;Moritz L;Sultan C;Gurczynski SJ;Moore BB;Tallquist MD;Li JZ;Hammoud SS
通讯作者：
Hammoud SS
5-羟甲基胞嘧啶在小鼠精子发生过程中的动态
影响因子：
16.6
作者：
Gan, Haiyun;Wen, Lu;Tang, Fuchou
通讯作者：
Tang, Fuchou
影响因子：
4.8
作者：
Bruscoli, Stefano;Velardi, Enrico;Riccardi, Carlo
通讯作者：
Riccardi, Carlo
DOI：
10.1126/science.1190614
发表时间：
2010-10-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Feng S;Jacobsen SE;Reik W
通讯作者：
Reik W