Relationships between cellular condensation, preosteoblast formation and epithelial-mesenchymal interactions in initiation of osteogenesis.
Relationships between cellular condensation, preosteoblast formation and epithelial-mesenchymal interactions in initiation of osteogenesis.
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成骨起始过程中细胞凝结、前成骨细胞形成和上皮间质相互作用之间的关系。
DOI：
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发表时间：
1995
影响因子：
0.7
通讯作者：
B. Hall
中科院分区：
文献类型：
--
作者：
L. Dunlop;B. Hall
骨生成或骨形成的起始依赖于细胞和组织的相互作用。我们研究了4和7天的孵育，翻译成成骨细胞和骨沉积在鸡胚下颌骨上皮间充质信号的明显分化之间的事件。在H.H.，用PNA-凝集素观察到下颌间充质（膜状骨骼）的浓缩。26中期（5.75天），持续12小时，比成骨细胞分化早1.5天。从三维重建确定所有下颌骨膜骨从一个单一的凝聚密切相关的造口上皮。下颌牙弓中的成骨凝聚是为上颌牙弓和下颌牙弓提供成骨间充质的常见凝聚的主要分支，这一发现建立了控制这两个牙弓中骨骼发育的机制之间比之前怀疑的更密切的联系。前成骨细胞（碱性磷酸酶阳性细胞）在H.H.早期25，其在凝结之前但在上皮-间充质相互作用之后，前成骨细胞的形成和凝结依赖于上皮-间充质相互作用-在分离的间充质中两者都不形成，而两者都在间充质和上皮重组之后形成。腱生蛋白仅在H.H. 19期，但在任何年龄都不在间充质中。因此，上皮-间充质相互作用控制了前成骨细胞阶段骨生成的启动。然后前成骨细胞凝聚，转化成骨细胞并存款骨基质。前成骨细胞的分化先于浓缩，浓缩放大了它们的数量。这与其中冷凝触发成软骨细胞前分化的软骨发生相反。
Initiation of osteogenesis or bone formation is dependent on cell and tissue interactions. We investigated the events between 4 and 7 days of incubation that translate epithelial-mesenchymal signalling into overt differentiation of osteoblasts and deposition of bone in the mandibles of chick embryos. Condensation of mandibular mesenchyme (the membranous skeleton), visualized with PNA-lectin, occurred at H.H. mid-26 (5.75 days), lasted 12 h and preceded osteoblast differentiation by 1.5 days. As determined from 3D-reconstruction all mandibular membrane bones arose from a single condensation closely associated with the stomodeal epithelium. The finding that the osteogenic condensation in the mandibular arch is a major branch of a common condensation that provides osteogenic mesenchyme to both maxillary and mandibular arches establishes a closer link between mechanisms controlling development of the skeleton in these two arches than previously suspected. Preosteoblasts (alkaline phosphatase-positive cells) form in the mandible at H.H. early 25, which is before condensation but after the epithelial-mesenchymal interaction upon which preosteoblast formation and condensation depend--neither form in isolated mesenchyme, whereas both form after recombination of mesenchyme and epithelium. Tenascin was present in the mandibular epithelium only at H.H. stage 19 but not in the mesenchyme at any age. Therefore, the epithelial-mesenchymal interaction controls initiation of osteogenesis at the preosteoblast stage. Preosteoblasts then condense, transform into osteoblasts and deposit bone matrix. Differentiation of preosteoblasts precedes condensation which amplifies their number. This is in contrast with chondrogenesis where condensation triggers prechondroblast differentiation.
影响因子：
7.5
作者：
G. Stein;J. Lian;T. Owen
通讯作者：
G. Stein;J. Lian;T. Owen
影响因子：
10.5
作者：
BLESSING, M;NANNEY, LB;HOGAN, BLM
通讯作者：
HOGAN, BLM
检测前软骨、胚基特异性标记物。
影响因子：
2.7
作者：
Aulthouse,AL;Solursh,M
通讯作者：
Solursh,M