Comparison of Schmallenberg virus antibody levels detected in milk and serum from individual cows.
Comparison of Schmallenberg virus antibody levels detected in milk and serum from individual cows.
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DOI：
10.1186/s12917-015-0365-1
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发表时间：
2015-03-11
影响因子：
2.6
通讯作者：
Blowey R
中科院分区：
文献类型：
--
作者：
Daly JM;King B;Tarlinton RA;Gough KC;Maddison BC;Blowey R
施马伦贝格病毒（Schmallenberg virus，SBV）是近年来在欧洲发现的一种反刍动物病毒。酶联免疫吸附试验（ELISA）通常用于检测散装罐牛奶样本中的SBV特异性抗体，以监测牛群感染情况。然而，先前已经表明，即使牛群中的大多数奶牛对SBV抗体呈血清阴性，散装罐乳样品也可以检测为阳性。开发笔侧测试来检测个体乳汁样品中的抗体可能会提供比通过ELISA检测个体血清样品更便宜的测试（其样品是非侵入性获得的）。因此，本研究的目的是研究牛奶和血清中测得的抗体水平之间的一致性。使用市售的间接ELISA检测了来自英国两个奶牛群中88头奶牛的相应牛奶和血清样品中是否存在SBV免疫球蛋白G抗体。还进行了血清中和试验（NT）作为金标准试验。散装罐乳汁样品获得的ELISA值与各牛群个体乳汁样品的平均值一致（牛群A和B的散装罐乳汁值分别为58%和73%，个体乳汁平均值分别为50%和63%）。在NT检测的88份血清样本中，82份（93%）呈阳性。尽管在较高的抗体水平下，单个乳汁样品的ELISA值往往高于相应的血清样品，但乳汁样品的阳性预测值为98%，血清样品的阳性预测值为94%。血清ELISA更可能在测定的下限临界值附近给出假阳性结果。结果表明，通过ELISA检测单个牛奶样品中的SBV抗体可用于为奶牛群管理决策提供信息，例如接种哪些动物（如果有的话）。
Schmallenberg virus (SBV) is a recently emerged virus of ruminants in Europe. Enzyme-linked immunosorbent assays (ELISA) are commonly used to detect SBV-specific antibodies in bulk tank milk samples to monitor herd exposure to infection. However, it has previously been shown that a bulk tank milk sample can test positive even though the majority of cows within the herd are seronegative for SBV antibodies. Development of a pen-side test to detect antibodies in individual milk samples would potentially provide a cheaper test (for which samples are obtained non-invasively) than testing individual serum samples by ELISA. Therefore, the aim of this study was to investigate the agreement between antibody levels measured in milk and serum. Corresponding milk and serum samples from 88 cows in two dairy herds in the UK were tested for presence of immunoglobulin G antibodies to SBV using a commercially-available indirect ELISA. A serum neutralisation test (NT) was also performed as a gold standard assay. The ELISA values obtained for the bulk tank milk samples corresponded with the mean values for individual milk samples from each herd (bulk tank milk values were 58% and 73% and mean individual milk values 50% and 63% for herds A and B, respectively). Of the 88 serum samples tested in the NT, 82 (93%) were positive. Although at higher antibody levels, the ELISA values tended to be higher for the individual milk samples than for the corresponding serum samples, the positive predictive value for milk samples was 98% and for serum samples 94%. The serum ELISA was more likely to give false positive results around the lower cut-off value of the assay. The results indicate that testing of individual milk samples for antibodies against SBV by ELISA could be used to inform decisions in the management of dairy herds such as which, if any, animals to vaccinate.
影响因子：
2.1
作者：
Loeffen W;Quak S;de Boer-Luijtze E;Hulst M;van der Poel W;Bouwstra R;Maas R
通讯作者：
Maas R
影响因子：
1.5
作者：
Ohlson, Anna;Blanco-Penedo, Isabel;Fall, Nils
通讯作者：
Fall, Nils
影响因子：
3.3
作者：
Veldhuis, A. M. B.;Carp-van Dijken, S.;van Schaik, G.
通讯作者：
van Schaik, G.