Expression of βA3/A1-crystallin in the developing and adult rat eye.
Expression of βA3/A1-crystallin in the developing and adult rat eye.
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DOI：
10.1007/s10735-010-9307-1
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发表时间：
2011-02
影响因子：
3.2
通讯作者：
Sinha, Debasish
中科院分区：
文献类型：
--
作者：
Parthasarathy, Geetha;Ma, Bo;Zhang, Cheng;Gongora, Celine;Zigler, J. Samuel, Jr.;Duncan, Melinda K.;Sinha, Debasish
晶体蛋白是晶状体中非常丰富的结构蛋白，在其他组织中也有表达。我们之前已经报道了大鼠βA3/A1-晶体蛋白基因的自发突变，称为Nuc1，具有一种新的、复杂的眼睛表型。本研究采用原位杂交和免疫组织化学方法，比较了野生型和Nuc1大鼠眼部发育过程中该基因的表达模式。βA3/A1-晶状体蛋白在胚胎第12.5d在野生型和Nuc1大鼠眼内均有表达，且在整个胚胎发育过程中一直局限于晶状体纤维中。出生后，βA3/A1-晶体蛋白在神经视网膜(特别是星形胶质细胞和神经节细胞)和视网膜色素上皮中也有表达。这表明βA3/A1-晶体蛋白不仅是晶状体的一种结构蛋白，而且在眼的其他组织中也具有细胞功能(S)。综上所述，βA3/A1-晶体蛋白在不同的眼组织中的表达受到不同程度的调控，其中晶状体是表达最高的部位。ISH和IHC在野生型和Nuc1动物中检测到的类似染色模式表明，Nuc1蛋白的功能差异，而不是mRNA/蛋白质表达水平的变化，可能是Nuc1发育异常的原因。
Crystallins are very abundant structural proteins of the lens and are also expressed in other tissues. We have previously reported a spontaneous mutation in the rat βA3/A1-crystallin gene, termed Nuc1, which has a novel, complex, ocular phenotype. The current study was undertaken to compare the expression pattern of this gene during eye development in wild type and Nuc1 rats by in situ hybridization (ISH) and immunohistochemistry (IHC). βA3/A1-crystallin expression was first detected in the eyes of both wild type and Nuc1 rats at embryonic (E) day 12.5 in the posterior portion of the lens vesicle, and remained limited to the lens fibers throughout fetal life. After birth, βA3/A1-crystallin expression was also detected in the neural retina (specifically in the astrocytes and ganglion cells) and in the retinal pigmented epithelium (RPE). This suggested that βA3/A1-crystallin is not only a structural protein of the lens, but has cellular function(s) in other ocular tissues. In summary, expression of βA3/A1-crystallin is controlled differentially in various eye tissues with lens being the site of greatest expression. Similar staining patterns, detected by ISH and IHC, in wild type and Nuc1 animals suggest that functional differences in the protein, rather than changes in mRNA/protein level of expression, likely account for developmental abnormalities in Nuc1.
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